Hello everyone! I’ve been curious to know if there are others out there who are in my position and feeling the overwhelming work load a lab can bring to an individual/team.

I’m a lab supervisor for a biotech company and the work load has increased double what it used to be but with the same amount of people we’ve had for years. The higher ups tell us there’s going to be an increase next year for the company but that means we will work even more than we already are (10+ hours of OT per week). Does anyone else feel burnout from their lab? Is this normal? Is there another option for someone who studied Biochem that’s NOT lab work? I’d love to hear any and all options if you have any!

Hi!! I’m taking my first intro to biochemistry course and I’m not doing that well. I really want to work to do better in this course, and I was hoping if anyone had any links to perhaps practice problems or supportive videos on YouTube etc that will help solidify concepts please. Also, advice from anyone with more experience in the field would be appreciated!!

How useful to you all would a physical cell lysis tech be that: does not generate heat and can pellet cell debris in one step? Basically like a spin tube that can lyse cells and pellet at the same time. You could use whatever buffer you like, since it’s physical no lysis buffer would be needed.

I created a subreddit (r/ORISE) yesterday for this niche group to share application tips, talk stipends and benefits, network across different fields, and get career advice. Whether you’re just starting or a seasoned fellow, we’re here to support each other!

I‘m trying to quantify the amount of chromatin in a nuclei solution, that were isolated from plant.

I want to use equal amounts of chromatin from different isolations for multiple enzymatic digestions.

Did any of you do something like this before and how did you do it? I am trying to perform phenol-chlorophorm extractions followed by A260 atm, but results are unerliably far from each other.

Hi, I know /r/ biochemistry exists but it is a much smaller community.

I'm reading about ketoacidosis for fun and originally when I was reading, it seems to be that ketone bodies are produced as conjugate bases and it's not that acidic molecules are produced that immediately dissociate to their conjugate base form.

This view seems to be supported by this one paper https://www.cell.com/trends/biochemical-sciences/abstract/S0968-0004(19)30005-2

However, in this paper they cite a Robergs (2004) paper on lactic acidosis which seems to have been debunked by multiple responses by other authors.

Both papers argue that our simplified reasoning for acidosis (ketone bodies/lactic acid production) are flawed in that the acids themselves are not produced but that the decrease in pH comes from protons produced in prior intermediate steps.

I'm a bit unsure how to weigh this?

Im preparing for my enrollment in biochemistry and wanted to ask whether a laptop is fine for taking notes or are an ipad/ handwritten notes needed for jotting down chemical structures etc. Pretty uninteresting question but I don’t know where else to inform myself. How do/did you go about it?

I am using a RFP and BFP to look at E.coli cells with an AAA+ protease and cells without, and have found that the fluorescence intensity is always higher in the cells not containing the protease. Is there a way I can determine that the fluorescent proteins are substrates and get broken down or whether it's a coincidence?

Have got access to protease structure (and possibly the FPs but will have to check) along with PyMOL software

Also, how would you recommend investigating changes in fluorescence over time when I mix the two (will be 0.1%-1% mutant and 99% wild-type) to look at whether the mutant is 'cheating'?

I’m in my first year of biochem and i’m about to get 3 As and 3 Bs (the As are in my major classes) and I feel extremely anxious and disappointed in myself about it can someone tell me if you need to have a crazy good GPA for grad school or if i’m totally screwed?

Trying to decide what classes to take?

Want to know what the job outlook is with a biochemistry degree?

Trying to figure out where to go for graduate school, or where to get started?

Ask those questions here.

I’m currently studying biochemistry for a hobby. I’ve currently got a university text book and want to know what math skills I’ll need to know. For example calculus

(Note: As I am posting similar messages in other relevant subreddits, you may encounter similar inquiries if you participate in biology and chemistry-related subreddits.)

Recently, I came across James A. Peters' "Classic Paper in Genetics". Obviously it was a much more curated and professional selection but if you were to compile a list of the most significant and pertinent papers in Biochemistry, which ones would you select and why?

I'm getting my bachelors this April and I want to get a PhD and go into research. I've already got a strong academic and research background that could probably get me into a reputable program, however applications are due in a couple weeks and I haven't had time to start them and I don't think I will. Would doing a post-bacc make sense? What is a post-bacc like and how much do I pay or get paid?

We've been learning about glycolysis over the past week and one of the mechanisms for control highlighted to us was the effect of AMP on PFK. Our lecturer said that AMP is a particularly sensitive indicator of energy requirements in the cell because of the reaction catalyzed by adenylate kinase

2ADP <=> ATP + AMP, K~1

The explanation for this was that [ATP] = 10[ADP] = 100[AMP], so a 10% decrease in [ATP] causes a 400% increase in [AMP] and 100% increase in [ADP]. It's this bit I'm having trouble understanding - is there any mathematical, worked example to show this?

Intuitively, it kind of makes sense that [AMP] would increase disproportionately because [AMP] is so low to start with so even a small absolute increase is a large percentage increase, but why does [ADP] increase too if equilibrium has shifted right as a result of the decrease in [ATP]? I've tried working out an ICE table for the reaction but I haven't gotten anywhere, not even sure if you can use ICE tables to figure this out?

I just switched my major from Psychology to Biochemistry (massive change, I know). Not only am I extremely fascinated by the field, but I am absolutely DETERMINED to prove to my doubtful family members that I am NOT in over my head with this switch (even if I am, just a little). I've already bought a few books on Biochemistry, Organic Chemistry, Microbiology, etc, and I want to just dive into the study as best as I can so I can start next semester knowing what I'm getting into.

So, here's my question for those of you who are well-versed in the subject/major: In your opinion, what are the key points of Biochemistry? What are the most fundamental building block facts that carry the whole thing on their backs? What are some things that I've got to know if I'm ever gonna be able to call myself a Biochemist?

I have been searching for solutions for my dog's arthritis. She just turned 15 years old. She is about 50 pounds, mixed breed dog. Still loves to hike. Also does water treadmill therapy three days per week. We have been using a supplement from Cannanda called CB2, so I have been researching into how it works and comparing it to any other potential CB2 agonists. Everywhere I have been reading says beta-caryophyllene is a CB2 Agonist. Today however, I came across a study saying it is an Inverse Agonist. https://www.longdom.org/open-access/cb2-receptor-binding-affinity-of-various-nutraceutical-ingredientsand-their-combinations-75852.html. "For the agonist assay, the individual ingredients with a strong stimulation effect for CB2 receptor binding were Acmella oleracea (78.5%), cruciferous vegetable blend (59.1%), and two of the market brand CBD oils (52.5%, 50.5%) (Figure 2). The only compound with a weak to moderate effect was Sichuan pepper (25.9%). The copaiba essential oil showed a strong inverse agonist effect (-125.7%). This test was primarily enacted as a means of screening individual ingredients with potential for CB2 receptor binding in a multi-ingredient formula. However, relatively small standard error values with only having duplicate measurements add confidence to the results of binding effects."

What do you think about this? Is this truly conflicting information, or am I interpreting something wrong here?

Thank you.

Hey everyone! I’m a sophomore majoring in biochemistry and have no idea what career path I’d like to go down. I’m considering med school, or getting an accelerated BSN after graduating, or possibly just going into research straight out of college, but I really have no idea still. As far as double majoring, I’m wondering what would be most marketable/useful to increase my odds of landing a job right out of school, if I decide to go that route. Right now I’m leaning towards either double majoring in economics or getting a certificate in Business or Health Policy. Any thoughts or personal experience on this?

Currently 3 years into a BS in Biochemistry and I still have no idea what I’m doing. This wasn’t my dream, not my passion, and I don’t even know what job I’d get if I graduate. I’m not gonna lie, I’ve only been doing this to satisfy my family. That their son has a fancy degree title they can show off to their friends. The way things are going I might have to take an extra year to finish, and a master’s program might be unreachable considering my track record. My parents told me I have to work a job I hate in order to be successful, but I’m not sure they’re right anymore. Am I supposed to feel this much dread and sadness?

If there’s any chance I can rebound from this I’ll take it because it’s the only option I have left. I gave up my dream of being an artist for this, I feel like I’m too far in to stop. Sorry for the rant but I really don’t know who else to turn to.

Has anyone here ever tried to characterize human IgG on CE-SDS? I'm trying to tweak the assay and sample prep, but so far with everything i've tried, the main peak keeps showing up around the 240 KDa mark (where it's known IgG is ~150 KDa).

I've tried upping the SDS concentration, introduced short chain alcohol at 5%. I'm going to try SHS instead of SDS, but that reagent hasn't arrived yet. Even when I run the reduced assay, the heavy chain always appears higher than you'd expect, ~80 KDa instead of 50 KDa.

Trying to build a PC right now and I'd like to be able to do some structural biology processing on it. For the most part the heavy computing programs (like Cryosparc) are hosted on a dedicated cluster that I remote into. The only programs I run locally are Coot, Phenix, ChimeraX and some helper python packages like EMAN2.

As far as I know, CUDA cores are practically considered necessary for bioinformatics but what about the above listed programs? To be honest I don't even know how much these applications can take advantage of the GPU so I'm hoping someone here can weigh in. Ryzen GPUs are more accessible price wise for me so I'd prefer to do with one of those if possible.

If this is the wrong sub to post in please let me know where would be better and I'll remove this. Thanks!

Hi Good day!

Is there anyone here who can give advice as my raw crude extract is in semi solid state and I am having confusion whether to dillute it with another solvent in order to immerse the metal proceeding to weight loss method

short summary: I'm a first year, but I want to study medicine in the long term

any advantages and disadvantages of going to medical school same to biochemistry, and what do you think about the future as a biochmist

Iam a chemistry major and I did my masters in general chemistry but Iam always amazed at how chemical principles govern physiology.I have always loved learning biology too so I was thinking of going for a PhD in systems biology so how hard do you think it will be and can you also talk about career prospects..I have some experience in comp chem.

https://www.nature.com/articles/s41598-023-37718-5

My understanding in this paper is that Magnesium Chloride Hexhydrate can be used as a transdermal delivery system?

Would this only work for hyaluronan or for other active ingredients as well?

I have already taken the weight of my added histag into account, yet after loading into sds-page it showed 17KDa rather than 14.3kDa(expected amount).Is it possible that his-tag interacts with sds in some way that makes it migrate slower than expected ? If not, can anyone share what other factors could cause this?

In the netherlands we dont have biochemistry as a bachelor. Bio-pharmaceutical science is the degree that i think comes closest to it and is only given at one uni in the whole country. Is it recommended over for example biomedical science? Im currently stuck between biomedical science and bio-pharmaceutical science. I think its going to come down to better job prospects but i honestly cant find much info about it here in the netherlands. Its a really niche degree here and I cant find much about the caree you can have with it here.
Any advice would mean the world!

I'm looking at areas to read/watch videos about for my personal statement for university, and I'm wondering what would be really interesting to look at.

I have already looked at CRISPR-Cas9, but I reckon that's a bit too generic and the admissions team have probably read thousands of statements on it. Not sure where to look next.

Anyone have any recommendations?

Writing a paper?

Re-running an experiment for the 18th time hoping you finally get results?

Analyzing some really cool data?

Start off your week by sharing your plans with the rest of us. å

I’m currently studying biochemistry/biology right now mostly as a hobby and to cure my boredom. I’ve been thinking about getting a textbook and seems like I will be getting the ‘fundamentals of biochemistry’ 2nd edition. Is that any good?