Writing a paper?

Re-running an experiment for the 18th time hoping you finally get results?

Analyzing some really cool data?

Start off your week by sharing your plans with the rest of us. å

1. Why is CaCl2 used in the experiment?
2. What’s the difference between the amino acid structure of BFP and GFP? And when/why do scientists use one over the other?

Has anyone submitted an F99/K00 fellowship grant to the NIH? Hoping to submit my proposal to NIGMS for the October deadline and I’m using a modified F31 proposal. Any reccomendations or tips would be helpful, especially with aim 2 where you’re proposing your post doc work. Thank you!

Hi, Im still at the beginning of my educational/career journey. I just got accepted into biochemistry (undergrad degree) and Im aiming to get into medicine after my bachelor degree. If med doesn’t workout for me, I was wondering what jobs pay the most with a biochemistry degree. Even if it means that I need to obtain a Phd or a postdoctoral. Also, if it’s s a job that isn’t biochemistry but I can shift from with a bachelor in biochemistry I’m open to suggestions.

Thanks.

Hi everyone, I am currently a second year Biochem major and I’m interested in becoming a CLS after. I live in California and doing my own research and talking to others I have realized that CLS programs can be pretty expensive here in the area. Personally I don’t want to move to a different state to go to school and get my CLS certification. Which is why I want to get a job right after graduation to pay for the CLS program. I know nowadays most jobs ask for more than just a Bachelors degree, but I was wondering if there are any jobs I could apply to after graduating. Maybe someone else has been in my situation. I am currently working with a Dr from my school in a lab research. So I could also add that for my lab experience.

I am a first year undergraduate American biochemistry student in the UK with exams 5 weeks out. I have no idea what I am doing. I have no comprehension of chemistry whatsoever. I can hardly understand chemistry symbols, not one with more than 4 atoms. I cannot add chemical equations, I don't know what a benzene ring is. I ask my professors questions and they refer me back to more things in the past that I do not know. Help.

Does it affect the citric acid cycle? Shut it down, like would happen to your adrenal glands if you did steroids for too long? Or would it suck substrate from all other parts of the body and shut down other things in order to process the excess, like happens to your liver if you drink a bunch of alcohol? Does excess citric acid affect other parts of the body?

Since this happens in the mitochondria and the cycle eats ATP, how does this affect energy? If the cycle gets shut down, do you feel less tired since no ATP is getting eaten up? Or do you feel awful since this is the cycle that produces the de-oxidizer NADH, and you'd have none?

Help!

I'm stuck, as a current junior and rising senior, what would work best for me, if I wanted major in Biochemistry? There aren't many resources for me because I am low incomed, so any help would be great!

A pre college summer program or an internship(I'm already in, it's more biotech based, but its better then nothing)?

One of the paper says that "Immobilon-PSQ membrane with a 0.2 μm pore size suitable for proteins with a molecular weight less than 20 kD" while another paper published in natures says that "According to the results of Figs. 1, 2 and 3, with the increase of molecular weight, the binding ability of NC membrane to protein gradually decreased, while that of PVDF membrane gradually increased."

Is pvdf and immobilon psq the same ?

How to choose between NC and PVDF according to molecular weight of protein ?

We've just been taught thiamine deficiency can lead to several things, dry and wet beriberi being 2 important diseases. But my doubt was how is it decided whether a person will get dry or wet beri beri. Is it a matter of how intense the deficiency is? Or is there some other factor involved? Just to be clear this isn't homework, I asked this question to my medicine professor as well but he also wasn't sure about it.

What all simple projects I can plan using one of these two cell lines. The experiment should be of low cost and approximately 1 month period. Please help.

As a matter of course for my research project I purify insoluble proteins from bacterial inclusion bodies using a 7M urea buffer after initial lysis. The most recent protein I have worked on does not leave a solid pellet after extracting and then spinning down with this buffer, but what I can only describe as a "snotball" - a viscous mass of goop that is distinct from the regular supernatant containing my protein of interest but which doesn't pellet.

Any experience with this or explanations? Thanks.

Edit: want to clear up - this isn't a problem at all, I still get good yields of clean protein. I'm just curious.

Hello, student here, my biochemistry teacher has sent us this image of a molecule with the premise that we guess what type it is and justify our answer. Anyone who wants to give their opinion?

as a biochem student, what would be the best way to enter medicine research? I’ve been searching and looking at different paths, most specifically I’d like to enter some sort of neurobiology medicine research (dementia, Alzheimer’s, CJD). Currently I will be graduating with a BS, but, i know research is typically grad student level. I don’t have the typical undergrad research needed, but what would be some good jobs/things that I could do to obtain that? My end goal would to be a professor that teaches biochemistry, but also does research for the university, though i come from a very poor family whos never been in this field and I’m really struggling finding routes into this. Any information is appreciated!

Hi guys,

I've recently been doing intro biochem and wanted some clarification on the Hill Coefficient of hemoglobin. I know that the hill coefficient isn't the same as the stoichiometric ratio (i.e., nh=3, and n=4), but I don't really understand it. If anyone has an explanation, it would be much appreciated :))

Have you read a cool paper recently that you want to discuss?

Do you have a paper that's been in your in your "to read" pile that you think other people might be interested in?

Have you recently published something you want to brag on?

Share them here and get the discussion started!

Hello people, I'm graduating with a BA in biochemistry this summer and looking for a job. I'm considering taking a gap year before going to med school, so I would like to look for a job now. To gain a better understanding of salary, if I apply to a research assistant around Long Island, New York, how much I should expect for salary? I don't wanna be too cheap (my college in Ohio paid me 10$ per hour, but we all know that money in Ohio and New York are different). Will a 45k per year at the Cold Spring Harbor Lab a proper salary? I found several position in this lab and just wish to know more about salary before I apply

Pros: I have 3 years of lab working experience with many cellular experimental techniques (cell culture, cloning, plasmids constructing, transfecting, western blot, transformation, maxi/mini-prep, etc). Cons: Never processed a mouse model (but willing to learn). Unfortunately my laboratory results are not good enough to be published.

I'm in the process of learning the steps in the citric acid cycle, and when looking at the cycle I was curious if certain intermediates can be straight up inserted into the cycle or if they need to go through the full thing in order to be used correctly?

For example, fumarate to malate via fumarase - if we consume food with malate in it and have a higher than normal concentration of malate in our body, will fumarase not need to be used, let alone all the other preceding steps in the cycle?

Actually I guess the downside of skipping all those steps would be missing out on all the NADH, ATP, and FADH2 that are produced beforehand.....but still curious if certain components can be inserted into the cycle?!

My only background on this topic is intro biochem so idek if this question makes sense

I cant stand it. This happens so much. Im basically seething in my chair reading these comments. I know these people mean no harm but they really do harm by spreading around misinformation like this. Antibacterial/microbial is NOT the same obviously 😭. This wont do anything against viral infection but they swear it ”works” like wdym your body is just fighting off the flu lol, garlic & honey or not.

Hello,

I'm working on an internship and one of my tasks is to create a list of college-level books that talk about careers in Chemistry and Biochemistry such as:

What kind of careers are in biochem?

How to do the jobs in such fields?

What tests are required for grad school?

Does anyone have any suggestions?

Hi!

Not sure where else to ask but I figured you guys might know. I’m studying for my country’s biochem entrance exams and I’m looking at old exams and I’m stuck on a question. I’ll try to translate accurately:

”Carbonic anhydrase catalyses the conversion of carbon dioxide and water into carbonic acid which in turn creates bicarbonate and hydrogen ions. The enzyme is quite fast, and its kcat=4,0•105s⁻¹ (reactions per second). How long does it take for the enzyme to process one substrate molecule?”

The answer is very simply written as ”t=1/kcat=1/4•105s⁻¹=2.5•10⁻⁶s=2.5 µs”

I don’t understand how this is calculated and the answer only leaves me with more questions. Can anyone help?

The question was asking something like which of the following best represents the net equation of the pentose phosphate pathway when 5 carbon sugars are needed.

The correct answer to the question should be: glucose-6-P + H2O + 2 NADP -> CO2 + pentose-5-P + 2 NADPH This is even in the slides. But this right answer was not one of the choices. Instead the choices were something like:

a. Wrong b. glucose-6-P + 2 H2O + 2 NADP -> CO2 + pentose-5-P + 2 NADPH c. Wrong d. Wrong e. None of the above

Answer b is also wrong because it says that the PPP uses two H2O molecules. Choice b. breaks the law of conservation of mass. Therefore, the correct answer should be e. None of the above.

It’s like asking “which of the following equations best represents the formula 2 + 1 = 3?” And the answer choices are:

a. 2 + 2 = 3 b. 2 + 2 = 5 c. 2 + 1 = 4 d. 2 + 1 = 5 e. None of the above

e. Should be the answer because none of the choices are accurate.

It doesn’t seem fair to me to lose points on a question where the answer choice is incorrect, and it breaks one of the fundamental laws of the universe.

My professor is not conceding. He says that besides the number of water molecules, that was the answer. Am I in the wrong in this situation? It seems like the number of water molecules should be crucial. I don’t know what to do at this point.

So I'm finishing a bachelors degree on Biochemistry and I'm a bit lost about my future right now. I don't know whether to start a masters degree and pursue a doctorate even though my grades aren't really good or start working right away. About the masters, I was thinking maybe Celular and Molecular Biology and get a part-time job to get a doctorate. If I don't get into a masters degree, what job could I apply for?? Thanks in advance.

I’m working on a biodesign project and would love to propose a new innovative idea. I’m well aware of the dimensions mycelium offers and its durability and flexibility on creating a great sources of alternatives for many environmental damaging elements. However, due to the constant reports on mycelium I would like to try to work with a new material that is similar to mycelium but IS NOT mycelium. I read and see about it everywhere I would just like to try and research something new but still just as dependable and innovative.

So basically what’s a good alternative or substitute for mycelium? Something that still has all the good attributes of mycelium?

Hello! I am a PhD student in biochemistry who is planning to master out. I haven’t been so happy in the program and feel like it’s time to leave. I am looking into mastering out next year once I am able. However I want to have a plan before i pull the trigger. I have decided that i do not wanna teach or work in a lab for the rest of my life. I am thinking to take more the business/ management route instead along with putting my masters into work. I was wondering if anyone has taken such route and what opportunities should i look mostly to?

Any job or position recommendations that I would he qualified for?