We learned in class that allosteric enzymes don't follow Michaelis-menten kinetics, rather they have a sigmoidal curve. But allosteric enzymes all have quaternary structure due to multiple subunits. Which begs the question, are there proteins out there without quaternary structure that do not follow the michaelis-menton model of kinetics?

Can you please suggest me some whats groups or instagram accounts where i can get the information about all the research programs and seminars especially related to chemistry and biochemistry.

I’m currently learning out chemicals contained in plants and I was wondering if there is a list or something of the current chemicals and acids and what not that are used for physical and mental health and what ones pose harm.

Hi all,

Which of these three crystal setting robots is the best? What are the pros and cons of each? I have only used the gryphon but am shopping for a new robot. I can see each having their benefits but i wonder what the community thinks.

I don't remember where I read this but I have a flash card with the following distribution for magnesium in the human body:

• 60% in bones (30–40% in hydroxyapatite, 15–20% interchangeable, god knows where the rest is)
• 20% in muscles
• 20% in blood cells and other tissues

The first place I checked was Wikipedia and magnesium isn't even mentioned once. From a quick Google search, I see that magnesium gets incorporated into hydroxyapatite, but how exactly does it work?

Hello everyone. I would like to know what specific ingredient in layer feeds that increase the performance of laying hens. I am student who is in the beginning of his thesis journey. It would be appreciated if someone answered.

Not sure if this is the right place to post this but is it possible to change the phenotype of an adult mouse (e.g., eye/hair colour) by injecting it with genetically edited cells, or can changing the phenotype of an organism only be accomplished during early embryonic stages ?

I just transferred schools to VT. I’m currently in chem 2 and bio 2, I have to take orgo 1 either way. I also did pretty well in other classes(physics 1, chem 1, bio 1, etc). The time to graduate would be the same for both degrees, but I don’t really see myself going to PT school anymore since it doesn’t seem worth it for the amount of debt. Would career outcomes be better in this field compared to exercise science?

Hello, I was wondering if any of you could know of any research regarding the development of math models ( kinetic models probably) for the dna extraction process. I am not sure if it sounds dumb, just that sometimes it might appear random at first glance the variation in methodology that are are shown in papers. Depending purely on empirical methods. I trust in your wisdom. Thanks for the time :)

Hey! I’m currently an undergrad biochem student at clemson, but am looking for help finding positions to do while in school in either a lab, anything healthcare related, biotech, etc. I am not certified in anything as it’s pretty expensive and hard to manage with my classes so it’s been hard to find things that work with this. Also, I graduate next may so even something that works as a stepping stone to build my resume would be great! Any help would be so appreciated

Trying to decide what classes to take?

Want to know what the job outlook is with a biochemistry degree?

Trying to figure out where to go for graduate school, or where to get started?

Ask those questions here.

My question is if you have a mixture of two proteins (A and B), and protein A has a His tag on the N terminus, and a binding pocket for protein B on the C terminus, if you run them on Ni-NTA resin, will protein B also stick? Or will it come off in the flowthrough?

Hello everybody. I'm currently writing my PhD thesis and need to write the Kd of the affinity of the complex nickel-NTA with a His6 on a protein. The problem is that I've found two very different values. In the first paper (older) they say 10^-13 M and in the second one 10 uM, which is quite different. Do you have any info on the subject? Thank you very very much in advance.

Papers:

1. https://pubmed.ncbi.nlm.nih.gov/8114690/
2. https://pubs.acs.org/doi/10.1021/bc900309n

Hi all,

As part of my research, I regularly need to pull down on biotinylated proteins with streptavidin-coated magnetic beads (the magnetic format works best with my workflow). I currently use the Pierce product with works super well--binding time is quite fast (10 secs yields complete binding) and high capacity, even unaffected by a small amount of free biotin in solution, and super fast precipitation when put against a magnet--but it is quite expensive, at ~$300 for 1 ml and over $1000 for 5 ml. I am wondering if anybody has compared these with other products that might be cheaper? For instance I see that the NEB product offers 5 ml for only about $400, although they quote a slightly lower binding capacity in the specs (and I don't know how responsive they are to magnetic stand). If anybody has any comparative info it would be super helpful. Thanks!

Writing a paper?

Re-running an experiment for the 18th time hoping you finally get results?

Analyzing some really cool data?

Start off your week by sharing your plans with the rest of us. å

Does anyone know if a deuterium nucleus (a proton + a neutron) could fit through proton pumps, ATP synthase, etc? Or could the neutron change it too much?

For some reason when doing spectrophotometery my lab partner and I kept getting negative readings expect for wavelengths around the absorbance peak.

For context we used the protein MNeonGreen and got the absorbance peak at 505nm.The data says it should be 506nm so we were pretty close despite the strange readings.

My lab leads said to just go with it but I have no idea why this happened.Can anyone help?

It would be really helpful if you guys can share some insights into the difference between these majors as I'm really conflicted right now. Thanks!

Does anyone know what software is used to present a protein cartoon like this...

https://preview.redd.it/40ydigkjkdfe1.png?width=636&format=png&auto=webp&s=a051457e341fd29efc0520f75a2da689a4ab5cc9

In that part where both ketogenic and glucogenic aminoacids are forming acetyl-coA for the beginning of the TCA cycle, what is it that makes them different from each other and puts one into glucogenic and one into ketogenic category?

Have you read a cool paper recently that you want to discuss?

Do you have a paper that's been in your in your "to read" pile that you think other people might be interested in?

Have you recently published something you want to brag on?

Share them here and get the discussion started!

I'm interested in plant biochemistry and I'm trying to break down what areas of metabolism most interest me. The main areas that I've listed so far are:

1. Energy (cellular respiration and photosynthesis)
2. Defense (secondary metabolites and alexins)
3. Building materials (amino acids and nucleic acids)
4. Redox (antioxidants and photoprotectants)
5. Storage (lipids and polysaccharides)
6. Signalling (phytochromes, cytochromes, hormones)

Any other areas?
So far I think I'm really interested in Redox and Storage. I'm really interested in antioxidants and how nature deals with oxidative damage.

Undergrad Bio major here! What is the difference between proteins, peptides, aminoacids and macromolecules? As far as proteins and peptides is it their function?? Or is there a specific length they have to be to be considered a protein vs amino acid vs peptide? And as for macromolecules arent those just like fats, sugars, etc.

This might be a naive question, but I was hoping I could get feedback about a drawing I did recently. I am an artist now, but my background is in biochemistry. I graduated college in 1998. My bachelors degree was in microbiology with a chemistry minor. My career was in molecular biology, however it has been many years since I worked in biotech. I don’t remember most of the things I learned in school. I draw from my head straight onto the paper. No planning. This drawing easily emerged, and feels adjacent to things I’ve come across in my schooling and/or my career, but I can’t remember how or why. It’s called “interactions”. Any thoughts? Wrong platform? Am I a little Rosalind Franklin esque or just up in the night? Thanks, Lauren