We are high school students conducting a study on the antimicrobial property of a plant(Strobilanthes reptans) through the disc diffusion method. We are having a bit of a trouble with our methodology for the control groups. We plan to use ampicillin as our bacterial positive control, amphotericin b as our fungal positive control, and ethanol as our negative control. We will be utilizing the powdered form of ampicillin and amphotericin b and then dissolving it with a sterile distilled water and after that soaking the filter paper disk with the controls, and then finally using the modified Kirby-bauer test. So the problem is that we don't know the amount of the powder, distilled water, and like the concentration we need to use to dissolve the control. We would greatly appreciate it if someone could help us!

So i recently completed my graduation in ZBC and have been preparing for the SSC exam for some months. However, as it's a government job with high competition, I'm now considering pursuing an MSc in microbiology as it at least provides some placement opportunities in pvt pharmaceutical and food technology companies etc, which is what I've heard from my seniors. And, like microbiology can offer some job security compared to solely preparing for SSC. But i spent half an hour researching the situation of life sciences in India and found that it's not particularly good and promising here as it seems if someone wants to persue it solely. So my questions are;

1. Can I prepare for government exams like SSC while studying my masters in microbiology simultaneously, since the college I’ve selected has a 70% attendance requirement, but regular leaves can be taken after making a good name in the department( that's what the professor there suggested) ?

2)if preparing for both simultaneously is not possible, then should I pursue a masters in microbiology or should I continue my SSC preparation with full focus at home by taking a gap year? Would that be advantageous or disadvantageous?

As a girl in India, becoming independent as soon as possible is my priority. I don't want to make any wrong decision this time. Feel free to suggest!

Hi guys i'm just here for a little advice about possible paths to head on with my qualifications, i have been working as a school science tech for a few years now but that isn't something thats really testing my abilities or something i would like to do long term, i kind of fell into it post masters .

I have a degree in biomedical science and a masters in infection and immunity from a good university uk based, but im struggling where to head and what fields to try and aim for to use these, i worked with TB during my time at uni and found it extremely interesting and was looking into a PhD on the topid but PhD programs seem almost impossible to acquire funding from bodies, and self funding is not really a valid path for me with lack of savings, responsibilities etc.

Is there anyone here from the UK working in the field who can offer a bit of guidance and maybe ideas as to how to enter the field and what particular roles to look out for :)

Thanks in advance !

I worked as a Microbiologist (Pharmacautical Microbiology QC lab experience from Gujarat) and currently looking job opportunity in California. Do I need any certification?Any suggestions how to do a job search in bay area? Community colleges, Career fair,Training and internship program? Please let me know. I tried calling recruiter and fill out several job application through the company website, career site (LinkedIn, indeed, zip recruiter) but no single interview call.

We avoid high temperatures such as dry heat prior autoclaving to maintain the nutritional value of the culture medium. Thus, preventing protein denaturing. However, how does autoclaving prevent protein denaturing and retain the nutritional value of the culture medium while killing all the microorganisms?

Hi! Is someone more enlightened then me, basically a layperson in microbiology, interested in explaining what the chance is of misinterpreting E. Coli as Pseudomonas Citronellolis in a human blood culture? Assuming the blood was analysed by a modern hospital lab in a Western country and there is no contamination. I ask the question out of curiosity and with zero intentions to utilize this information for anything practical irl. Thanks in advance.

hi !

can anyone suggest an alternative for cork borers to be used for an agar plug assay? it's hard to find quality and legit sellers in our area. i tried looking it up, but to no avail as well.

if you know of any tips or tricks to go about this, please chime off below! thanks!

So say we had a bacterial cell that was about the size of a large pumpkinb and we wanted to cut it open

Upon opening it up, we'll be fine inside how much just a bunch of liquid, jelly or something like organs

What about other single-celled critters, Moodle look like if they were gigantified and cut open

I’m taking a microbiology class currently and I’m pretty sure I brought home S. Aureus. My 5 month old baby had a scratch on this lip and the day after that lab it started developing into impetigo. It’s now still spreading and I’m so stressed out I’m thinking of every way it could have happened. He doesn’t go to day care I’m pretty sure the only way this could have happened is because of lab. I was careful and followed the aseptic procedure I’m thinking it must have gotten on my pen or lab notebook idk. What did i do wrong?

I'm looking for a microbiology tutor for college level microbiology.

https://preview.redd.it/7ot0c3wrbwld1.png?width=1280&format=png&auto=webp&s=a3b3de0b882768df8f7c6d51780046c246006a15

Title says it all. I'm having trouble understanding if what I'm looking at is bacteria or soil particles. This is a sample of some compacted clay from my backyard.

How do you learn how to identify what is what?