/r/microbiology

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The study of eukaryotes, fungi, protists, prokaryotes, viruses, and prions.

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Microbiology

The study of eukaryotes, fungi, protists, prokaryotes, viruses, and prions.

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Rules
  1. No Politics
  2. No Religion
  3. No Racism
  4. No Condoning of Violence
  5. Please remember to add a link flair to your submission
  6. Refrain from microbe identification posts. Unless you have more than enough pertinent information to identify it, the post will be removed
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/r/microbiology

148,422 Subscribers

1

Does Swiftcam work on M1 Mac?

I recently got a Swiftcam SC1003 10 mp Microscope Digital Camera. I am trying to find a way to view the images but I can not figure it out. Any help?

0 Comments
2024/11/08
15:58 UTC

2

bacterial concentration adjustment

i am currently trying to perform antagonistic activity test. but the issue is every paper is suggesting for swabing 10^8 CFU/mL concentration of bacteria. how do i adjust broth to this concentration and measure it?

5 Comments
2024/11/08
15:24 UTC

2

Help Cleaning Biofilm Residue Off Glass Flasks

Hi, does anyone know how to clean leftover biofilm off these flasks? They are being used specifically for a cell growth quantifier, which measures backscatter, so we don't want to use any cleaning brush. Liquids only. Scratches, even those that are too small to see, will affect the readings. We currently autoclave flasks after use, then soak them in detergent for up to/over 16 to 24 hours.

https://preview.redd.it/266ljt2owozd1.jpg?width=3060&format=pjpg&auto=webp&s=4e7772dd322c8a91f30103c09c3c2c2332ebcf43

5 Comments
2024/11/08
14:42 UTC

2

What is this contamination in my cell culture?

Pen/strep does not kill it. Medium is red/pinkish and clear.

https://preview.redd.it/9aow6utlvnzd1.jpg?width=4032&format=pjpg&auto=webp&s=3cbe32e39a6ac992e74b01f2528c20ad11631b14

Note the small elongated shapes everywhere st 40x zoom. I thought some bacillus species, but perhaps its a yeast?

https://preview.redd.it/sp6a72xvvnzd1.png?width=1079&format=png&auto=webp&s=04fe8acc90822b52632adf780f0cccca91600fd9

0 Comments
2024/11/08
11:15 UTC

1

Thesis Topic

I'm in search of a topic for my undergraduate studies, my area of interest is biotechnology, bioinformatics and antimicrobial resistance. I will really appreciate any recommendations

5 Comments
2024/11/08
06:02 UTC

4

Research from post 2001 anthrax attacks showing antimicrobial resistance?

Hi I'm trying to find some evidence that the mass use of PEP might have caused some amr to exist in the organism.

Or anything about amr that is related to bioterrorism.

I tried to find examples from operation seaspray on the serratia colonization but the country did not have amr surveillance at the time so there is very limited data. Thank you!!

7 Comments
2024/11/08
01:03 UTC

2

was told to incubate my plates right side up, am i cooked?

i put some liquid culture on a plate (10 microliters) and it wouldn’t freaking dry, and so they told me to just incubate them right side up. and i know this is a big no no because of the condensation contamination. what im worried about is measuring zones of clearance after flooding with grams iodine after incubation (i am measuring degradation not antibiotic resistance btw). will there even BE any zones of clearance with this or am i screwed? the best part is this is for a research project 💀 the plates are incubated at 10C, 30C, 28C and 37C and each have multiple bacteria “dots” of different species. bushnell haas media. and before you ask, i was literally told to do this by my instructor. idk if they just don’t care that much anymore or if there’s still i chance i could get usable results

15 Comments
2024/11/07
20:17 UTC

0

Antibiotic susceptible genes

We all know about antibiotic resisting genes but do we have a list of genes that make bacteria more susceptible yo antibiotic treatments? I googled it and nothing came up

7 Comments
2024/11/07
19:22 UTC

1

Environmental microbiology - Online certifications

Are there any specific online certification courses in environmental microbiology/molecular biology/metagenomics/cell biology?
Moreover, are there any specific online certification courses I should do in aspects of crude oil bioremediation? It could not be specific to microbiology and can deal with crude oil in general.

0 Comments
2024/11/07
14:45 UTC

0

What bacteria can you confidently ID solely from the gram stain in x 100 magnification?

I know that most bacteria cannot be confidently IDed without further biochemical tests and cultivation, however I'm wondering if there are any you could confidently ID solely from how they look under the microscope. I'm not just talking about the family like streptococcus but also specifically the species. If so, which ones?

17 Comments
2024/11/07
12:02 UTC

1

Becoming a Microbiology Instructor

I have a bachelor's in microbiology from the university of Florida. I'm interested in this online master's in microbiology they have. Can you become a microbiology instructor at a community College with such a degree or do you need to do an in person master's with a thesis? Thanks in advance.

0 Comments
2024/11/06
22:46 UTC

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