/r/microbiology

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The study of eukaryotes, fungi, protists, prokaryotes, viruses, and prions.

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Microbiology

The study of eukaryotes, fungi, protists, prokaryotes, viruses, and prions.

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  1. No Politics
  2. No Religion
  3. No Racism
  4. No Condoning of Violence
  5. Please remember to add a link flair to your submission
  6. Refrain from microbe identification posts. Unless you have more than enough pertinent information to identify it, the post will be removed
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/r/microbiology

132,866 Subscribers

2

statistical tools in antibacterial thesis

consider statistics a stem student's waterloo.

we're undertaking our undergraduate thesis studies and i don't know how to approach our statistical analysis. for context, we'll be investigating the antibacterial properties of fungal endophytes from L. parasiticum leaves against 2 GN and GP bacteria. the assay will consist of two parts, one disc diffusion and one broth dilution.

i already know that we're going to use percent occurrence but i have no idea where to go from there. our biostatistics professor said that we should use kruskal-wallis as our sample size is less than 30 and that we should also do a post hoc.

i have literally no idea with this. freaking statistics, man.

6 Comments
2024/04/29
04:49 UTC

2

how to know whether “amino acid degradation” is for cell mass synthesis or reducing equivalents?

hey guys! fourth year micro student here doing my honours.

my thesis is quite metabolism-heavy which, granted, i have never been a fan of, so never paid too much attention. but now i’m slightly stumped!!

i’m looking at a few eukaryogenesis theories and trying to figure out the metabolism of the asgard archaea that took part in them. if something just says “hydrogen-releasing Asgard archaeon able to degrade small organics” or “degrades amino acids to short-chain fatty acids and H2,” how am i supposed to know whether those organic/amino acids are for cell mass synthesis or reducing equivalents?

thank you!!!

1 Comment
2024/04/29
04:01 UTC

1

Cell simulator

Is there a software that represents fully a simple cell structure with its 10s of million of molecules? the question is related to representation in a certain moment. A follow up is for simulating reactions and change over time in some medium, even if for a fraction of a second.

If such knowledge is not available today, what is the smallest biological "element" that we can simulate today to the molecule level?

0 Comments
2024/04/27
13:02 UTC

1

Azospirillum Agar - Need help

As the title suggests, i am currently working with azospirillum bacteria(Nitrogen fixer). Now, i don't know what specific agar should i use for plate count. I've searched literatures and other used NFb media and added congo red dye and "agar" and i'm lost since our medical technologist said that azospirillum has specific agar to be used in combination with the Nfb media. Can anyone help me with this?

I am a chemistry student and this is my first time dealing with microbiology so i have no deep knowledge concerning the subject so any help would be appreciated. Thanks!

0 Comments
2024/04/27
10:43 UTC

3

Can you tell different cocci apart without a doubt?

What I mean is, can you without a doubt tell enterococcus, staphylococcus, and micrococcus apart by just gram stain?

Someone thinks it's micrococcus but says he doesn't know and I'm thinking you really couldn't always tell all these three apart since occasionally micrococcus doesn't form tetrads. Obviously this one didn't or it would be a more confident guess.

45 Comments
2024/04/27
03:52 UTC

16

Is it normal to have mold spores on your hands?

This is probably a strange question but I thought I’d ask. I recently started a job as a microbiology technician. During one of my trainings, my supervisor was showing me how to collect fingertip samples using agar plates and he had me use my fingers. He ended up incubating the plates and one of them grew mold. Everyone on our team saw it and acted kinda disgusted about it and it honestly made me feel pretty shitty and self-conscious even though I know I’m a very clean person. I know bacteria is present on everyone’s skin, but I’ve never heard the same about mold. So is this normal or were they right to treat me like I had just taken a dive through a dumpster lol?

10 Comments
2024/04/27
00:19 UTC

2

Unknown Bacteria Project help

Hey guys. I'm currently working on a project where my prof gave us 2 bacteria that we have to isolate and identify. Any help would seriously be appreciated because I only have a week left and can't figure out any tests to do. Our lab is super simplified and everytime I look up a test to do we don't have the materials for it. I've used the bergey's manual but it hasn't helped very much. Idk if this is the right sub to post this in but you all seem super educated on the topic so I thought I'd try.

Heres my first guy and the tests I have done so far:

I believe it's either Pseudomonas or Alcaligenes, and my prof has helped me narrow it down to those two. I literally don't know how to differentiate between the two.

Gram negative bacilli

Oxidase positive

Glucose Ferm Negative

Lactose fermentation negative

Motile

aerobe

Citrate Positive

https://preview.redd.it/tdljg8rhvvwc1.png?width=2268&format=png&auto=webp&s=9eaa18c59e39d57173f724ee3d06d60195702ab4

My other one:

Either Aeromonas or Vibrio

Gram negative bacilli

Oxidase positive

Glucose ferm positive

Lactose ferm negative

Motile

Anaerobe

https://preview.redd.it/a9umnjfjvvwc1.png?width=2268&format=png&auto=webp&s=2d09bff930a983fc44597dac0b8313b063995d05

5 Comments
2024/04/26
20:31 UTC

1

looking for advice/help

hello im working on a study about the microbial activity of a local Philippine plant. I'm looking for someone who might have an idea/interest in the topic (preferably someone who has a published paper or knows of anyone of the sort). it's a pretty straightforward topic and the results were kinda... (spoilers). Anyways, just looking for someone interested or an expert on the topic! hihihi

If it sounds interesting or familiar please message me, or if you want/need to know more about what I'm talking about. any help is appreciated. thank yousssss : )

0 Comments
2024/04/26
17:16 UTC

0

Interesting microorganism for research paper

Im getting into the field and want to write a research paper to deepen my knowledge. The paper is probably going to be 12-15 pages long and I want to write it about a microorganism found in soil, water or the human microbiome and has a very distinguishing feature about it (maybe causes a disease) and that has enough literature around it that I could fill the amount of pages I want to write. I guess the volume of the information on it should be huge and it should be already well researched. Helping select one specific microbe would be much appreciated!

8 Comments
2024/04/26
13:54 UTC

0

Need a specific fungus for a report on how fungi decompose carcasses

Our Microbial ecology professor requested a report on a specific type of fungus that played a part of decomposing dead animals in the nature. Sure i searched everywhere but they say all fungi fungi fungi could play a role in this process. I need a specific type or species.

Please. I’m begging

2 Comments
2024/04/26
05:06 UTC

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