/r/Biochemistry

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A place to post news and discuss the frontiers of biochemistry and biotechnology. Please refrain from posting home videos with songs and raps.

/r/Biochemistry

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1

The potential of CRISPR to change healthcare. Thoughtful talk from Trevor Martin, CEO, Mammoth Biosciences

0 Comments
2024/06/11
14:39 UTC

5

How med labs actually test.

Hi,

Im actually in medicine, I grduated undergrad in biochem and its still the basis of my passion for medicine. - However, youd be surprised both doctors and students know which tests to order and when to order them (usually :/) but they have no idea whats happens after the check the box and sign the form. I was wondering if anyone knew (in high level, biochem language). Like after the blood/sample arrives at the lab. what methods are used for example to get d CBC with differential (that genneral blood test that tells us speifially the percentage of white blood cells, red blood cells, new cells, old cells, etc), or how can they differentiate hemoglobin A1c from other hemoglobin. Im not just looking for an answwer like "chromatograohy" i want too know what the preparation is, teh pretretament. if there is pre isolation/ separation or if whole blood/serum can just be tossed into a machine and it all works out.

THanks for any info/ insite in advance!

5 Comments
2024/06/11
12:54 UTC

2

Lactate as energy: waste products in urine or elsewhere?

If I may ask this question? If not please delete.

I have an inborn muscle condition, suspected metabolic myopathy. I do exercise a lot more than should theoretically be possible. I have periods where things don't work out at all, and work out surprisingly marvelously. Embarrassing bit: When running went marvelously my pee has an odd chemical sweetish smell and .. ahem.. taste, like diluted poor quality applejuice. Kidneys retain salt. This is not present apart from the lack of salt when running didn't work well but I still pushed through or gave up. I wondered whether this might be from muscle breakdown, but after 10 years of running and generally 20 years of exercising I still have very nice muscles (more than most despite rock bottom xx testo). It's also nothing that can be identified in a standard 11 field dip stick, thus likely not protein. No negative effects on kidneys/whatever yet.

There's a theory I'm pursuing and that I want to discuss with my next appointment in a muscle clinic: maybe my body is using lactate as source of energy at times. It's been described for McArdles disease and for severe mito. My reason: I get lactic acidosis if I do something too strenuous. Like if I do a slow jog and then run faster for 5 minutes, or try to run uphill. Compensatory breathing only kicks in some 2-4 hrs later though. But an exercise test with serial lactate measurement showed that my lactate was in fact quite a bit lower than expected. Measurements unfortunately were stopped when the exercise stopped, thus no idea what happened thereafter.

Question is: if you metabolize lactate as energy would there be anything in urine that can be identified, and how? Would this even appear kind of sweetish?

3 Comments
2024/06/11
08:27 UTC

7

I am studying about proteins, the basics. There's something that's confusing me, which is the meaning of functional protein

I was studying about the Quaternary structure of protein. In my text book, it says, ⭐'Aggregation of 2 or more polypeptide chains involve in the formation of one functional protein. Seperate chains are called protein sub units which were held together by intra and inter molecular interactions. Eg:- Heamoglobin, Collagen'⭐

My doubt here is, what do they mean by functional protein here? Isn't collagen a structural protein? Or did they mean 'its a protein that's capable of functioning' by the term functional?

3 Comments
2024/06/11
07:44 UTC

2

Trying figure out my academic path

Hi, I just graduated with a B.S in environmental studies and I am interested in pursuing a career in biochemistry. Is it possible for me to apply to a masters program in biochem directly? Should I get a B.S in biochemistry first?

0 Comments
2024/06/11
06:24 UTC

7

Chymotrypsin Specificity

Undergraduate student taking Biochemistry. My professor said that Chymotrypsin binds only to one large hydrophobic residue such as Phe and the residues adjacent to Phe do not impact Chymotrypsin's specificity at all. However, they also said that Chymotrypsin will cleave a substrate that includes the sequence Ser-Phe-Gly-Ser more readily than a substrate that includes the sequence Ser-Gly-Phe-Ser (where both sequences are part of longer polypeptides and are shown N -> C terminal).

These two statements seem contradictory to me. Does the presence of glycine as opposed to a polar amino acid at the C terminal of Phenylalanine make Chymotrypsin more likely to cleave that sequence of amino acids? Am I missing something else? Any help would be appreciated.

5 Comments
2024/06/10
20:23 UTC

1

Jun 10: Weekly Research Plans

Writing a paper?

Re-running an experiment for the 18th time hoping you finally get results?

Analyzing some really cool data?

Start off your week by sharing your plans with the rest of us. å

0 Comments
2024/06/10
15:00 UTC

1 Comment
2024/06/10
13:13 UTC

9

Where can I start getting experience in the field after high school?

So, I’m about to graduate from high school in a couple days. And I really want to start getting some experience in the career. Where should I start? I currently work at Walgreens but just up front, but I wanna try another part time job that can introduce me more to chemistry or biology

So any jobs or companies that you guys can recommend?

Thanks in advance!

6 Comments
2024/06/10
03:02 UTC

3

Cytochrome p450 expression issues

Our lab is working with bacterial cytochrome p450 heterologously expressed in E. coli. I can’t seem to express any functional CYP. I don’t see the characteristic red color after IPTG induction. I wonder if the issue is heme production (supplemented with 5-aminolevulinic acid). Any suggestions or tips you can provide would be appreciated.

7 Comments
2024/06/09
21:24 UTC

8

How can I verify if the dialysis has been successful?

As the title says, I am working on the dialysis of a protein that I have extracted from milk using two-step precipitation with ammonium sulfate at 45% then 80%, I am using distilled water as the buffer for the protein and the dialysis. I changed the buffer every two hours, 3 times, then I ran it overnight. How can be sure that I have gotten rid of salt from my sample?

After dialysis, I am trying to lyophilize the protein until further use. Then rehydrate it in a buffered saline solution to use it with a rodent model.

7 Comments
2024/06/09
20:30 UTC

3

Can someone explain/refer a source that explains N(i) to N(i+1) connectivity in NMR studies?

Please.

2 Comments
2024/06/09
11:35 UTC

11

High Schooler wanting to do Biochemistry

I love biochemistry but I have 0 idea where to start, as a 9th [about to be 10th] grader most knowledge / resources I have is growing molds and staring at cells and protists all day. I mainly view millipede cells from dead [natural death] millipedes and the toxins they secrete. This is all pretty lame to do and I want to start with better experiments and stuff that will really get me more experience and grasp on the field. [Entomology stuff helps! I love entomology and detritivores, hence why my main focus is millipede anatomy since it's understudied]

14 Comments
2024/06/09
03:28 UTC

22

So, what's it like exactly, getting a degree?

TL;RD me not amazing at chem will biochem make me wanna kill myself

I'm seriously considering going into biochemistry, and I was wondering if this subreddit could give some insights into their experience with it (mainly about its difficulty).

I know I want to go to school and learn more before I have to work, and I'm kind of split between liking a lot of things but not being particularly good at any one thing.

That being said, I'm good at bio (duh, you just need to study) and im decently good at chem (sitting at a 90% average). I found cellular respiration and photosynthesis in biology facinating and I really want to know more stuff like that but what actually happens at the molecular level. But, I'm also worried. Despite being interested, that unit wasnt at all easy for me, and if biochemistry is as hard as my bio teacher says then is it a good idea? I might be forced to drop out or loose any potential scholarships, which would suck.

So, to anyone who considers themselves not AMAZING at chemistry but who still did a biochem degree, was hard work enough to pull you through? My alternative to biochem is probably statistics, I'm a fan of math.

Btw im in grade 11 in alberta if that information is relevant for gauging grade and particularities of a biochem bachelors program.

Thanks a bunch in advance!

12 Comments
2024/06/08
23:41 UTC

3

Subcellular Fractionation

Does anyone have a protocol for cellular fractionation they’re willing to share please? I would like to fractionate nuclei only from a range of breast cancer cell lines (MCF7 etc).

3 Comments
2024/06/08
22:09 UTC

19

I need help understanding something for college

I'm trying to understand this D and L thingy,

D-fructose

so in a nutshell (according yo my understanding just opening the book for first time and prof didn't explain anything)

the db and o thing depends on OH

if the oh is on right it's D and if it's on left then it's L right?

this d fructose, why is it d fructose not L?, the Oh is on keft

pls don't laugh it's the prof fault for not caring about us

6 Comments
2024/06/08
20:31 UTC

10

Is it important to read one standard book?

So i have done some topics from Lehninger and some from Stryer. For some chapters I've turned to Garret and Grisham, also Lippincott. SO I have studied from many separate books but now I'm wondering if that was a bad approach. Share your thoughts?

3 Comments
2024/06/08
17:35 UTC

2

best universities in us and uk for biochem undergrad

i am having a really hard time deciding which to apply for read all their websites what do yall think??

2 Comments
2024/06/08
17:28 UTC

16

Dress code for the FEBS congress

I'm attending the annual FEBS congress, this June in Milano, Italy, and being a Ph. D. student, it's my first conference this big that I'm visiting. What is the expected dress code for such an event? Is semi formal ok? I know that many scientists aren't good at dressing and will be wearing T-shirt and shorts, but I don't want to be like that. I was thinking of beige chino trousers, light blue shirt, black blazer, cognac oxford shoes and Seiko driver's. For the less formal program (traveling, walks in city etc.) I would go with beige jeans shorts, cotton checked shirt or polo-shirt, comfortable sneakers and university cap. I'm not attending the gala dinner. Do you thing it's suitable outfit for such event?

I'm adding photo of the outfit I've described, but it's from a much smaller conference.

9 Comments
2024/06/08
10:46 UTC

3

D & L forms

Our instructor told us, in carbohydrates D and L form depend on the position of the OH on the last chiral carbon. If it's on the right, it's D and on the left, it's L.

She also stated that they are mirror image of each other but if we simply change the position of OH on the last chiral carbon and leave the other OH unchanged, there'll be no issue.

So, we just have to change the OH on the last chiral carbon in the cyclic structure except it doesn't have OH on the last chiral carbon, as the H from the hydroxy group joins with the =O of the first carbon.

If I leave the position of all other -OH unchanged in this case, then wouldn't the structures look exactly same?

2 Comments
2024/06/08
09:52 UTC

1

Why is my transfer buffer ice cold after western blot transfer.

Usually when my transfer is done the transfer buffer is warm, but recently when I’ve been plugging in 3 different tanks into the machine my transfer buffer is always ice cold after transfer. Ponceau stain still shows plenty of protein but my images are coming out faint. Is this the issue? If so how can I fix it? Thanks for the help in advance !

2 Comments
2024/06/08
04:46 UTC

3

Jun 08: Cool Papers

Have you read a cool paper recently that you want to discuss?

Do you have a paper that's been in your in your "to read" pile that you think other people might be interested in?

Have you recently published something you want to brag on?

Share them here and get the discussion started!

0 Comments
2024/06/08
00:00 UTC

13

What are my job options after a masters?

Sorry if this has already been asked before, but im wondering what the best career options are now that ive finished my masters. Based in Quebec Canada.

Basically, I did bachelors in biochemistry, followed by a masters in biochemistry with research and a thesis. I have 4 years of in lab hands on experience. I definitely know that a PhD is not for me since I’m not able to think outside the box in order to make new discoveries. And anyways a PhD here is 7+ years and I’m already 26, so I’m definitely looking to start getting into the job market and actually make money.

Ideally, I would like a high income position, but I honestly have no idea where to start looking for any of those. I feel like every time I look on Google or indeed or any job search websites I’m not able to find any high paying positions, it’s literally just biochemistry tutoring and PhD offers.

I am highly considering learning programming such as python and others (please indicate if you recommend any programs) in order to have more bioinformatics skills. I am also considering maybe going the data scientist/data analyst route but I’m not sure about that since I don’t think I would be using any of my education in that role.

I am also interested in remote jobs as I would like to travel quite a bit before settling down and starting a family so a job that all allows me to do that would be quite rewarding. But again, I have no idea what kind of jobs in biochemistry can be done remotely.

Long store short: finished masters in biochemistry, does not want to do PhD looking for high-paying job, possibility of remote(?), Please list job titles that would correspond and where I could find these positions, thank you.

7 Comments
2024/06/07
22:59 UTC

8

30 days study plan

Hey guys, so my metabolism final is in 30 days and I've pretty much forgotten everything. I need to know all the pathways, extremely small details, rematch the lectures and pp notes… I also work two jobs and can sometimes be distracted or unmotivated. What would you suggest for me to pass since this is 60% of my grade. What should be my routine for these days. I want to concentrate but with my ADHD and everything it's sometimes difficult so what would you do in my place? Thanks in advance.

9 Comments
2024/06/07
21:47 UTC

1

Elementary book PDF on-line

Is there elementary textbook for biochemistry i mean Lab work

1 Comment
2024/06/07
17:37 UTC

6

Undergraduate Student Looking for options

Hi everybody. I'm going into my senior year as an undergrad biochem major. Freshman year, I was on the pre-med track. I came to college not expecting how rigorous the coursework would be. I failed and re-took some courses. I admit this was totally my fault and I had other issues during my previous years in school that I'm still dealing with. The pre-med dream is over; I do not think I will get into Med school. I switched to pre-PA but also doubt I'll be admitted into a PA program. I know what you're thinking. Why didn't you just switch your major? I couldn't. My GPA was not high enough to switch to a different field. By the time it was, I had already completed most of my degree courses. Now I potentially thinking of getting a master's (I want to work in healthcare so Healthcare Admin or CAA are areas I'm interested in). However, because of the setback I have had I will not be able to take anatomy for an Anesthesiologist Assistant Program. Will this be vital in my application? I could try to take a summer online course but what if that won't aid in any way? For most programs I have found online note that A&P I and II or required or they'll say Anatomy w lab etc. Has anyone taken this route before and could give me some advice? I have also thought about not pursuing a graduate program and going to work. The issue is I don't have much experience working (I've shadowed and volunteered but am having trouble finding a part-time job). I've been looking into cosmetic chemistry and pharmaceutical jobs but I was wondering if anyone had some input on those fields as well. I do not want to pursue a PHD or do research. I've come to this thread for help. I don't have any family members who work in healthcare or know anyone who has struggled the way I have. Any advice would help.

3 Comments
2024/06/07
12:47 UTC

0

CuSO4

Ypd with copper

Hello Does anyone can help me maybe ?

I need to prepare some agar medium with different concentration of copper (CuSO4) from 0 to 6 mg/L. I was preparing a stock solution of 1 mg/L and then make the dilutions with the media but maybe it’s not the best solution. What is the best way to prepare it in order to get more accurate results? Thank you:)

6 Comments
2024/06/07
07:55 UTC

67

I can't find a job and I'm miserable

Hi everyone,

I am 29 years old, I have a BS in Biochemistry with a masters in Biostatistics and I have been unable to find a job for quite some time now. I've applied to countless of places since I finished my masters (its been 2 years) and haven't got a return offer still. I support myself by being a private chemistry and biology tutor so I am in touch with the subject. I have been thinking about doing a PhD as well, but I HATE lab work.
I am situated in Europe, also am an EU citizen.

Any advice you could give me would be highly appreciated!

42 Comments
2024/06/06
23:16 UTC

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