https://preview.redd.it/l6dglu4yiuzd1.png?width=628&format=png&auto=webp&s=ef9c888d328a40c255ec1061fa83eb22662d7a42

is it a text error? shouldn't NTP form?

I'm interested in using lavender oil aromatherapy for depression but have some concerns about its potential hormonal effects. I've read studies linking lavender oil to hormonal issues and premature breast growth due to its effects on estradiol. Since too much estradiol in males can also worsen depression I worry that this aromatherapy might have a detrimental effect long-term.

I would only be using it through inhalation (few drops on a cotton pad, breathing in for about 20 mins daily) - NOT applying to skin or ingesting.

My question: Do these hormonal effects only happen with skin contact/ingestion, or can they also occur through just breathing in the scent?

Would really appreciate any insights or experiences.

Hi, can you help me understand this figure? I'm just confused about the arrows extending from the VLDL/LDL to Mature LDL and vice versa with things like CE and TAG written on them. Does this mean that when the two lipoproteins are within each other's vicinity there'll be an exchange of Cholesteryl esters towards the Mature HDL, and of TAGs towards the VLDL/LDL?

This is the only part that I'm having problem with this topic, and any insights would be really appreciated!

Have you read a cool paper recently that you want to discuss?

Do you have a paper that's been in your in your "to read" pile that you think other people might be interested in?

Have you recently published something you want to brag on?

Share them here and get the discussion started!

I am currently in my third year of undergrad, and I want to do an industry internship this summer. I have found it hard to find companies hiring biochemistry students within Canada, and it seems most US companies prioritize US citizens and students. Does anyone know Canadian companies or what type of companies in Canada would be hiring in that field?

Also, if I did research at my lab, I think I would have a high chance of getting an NSERC, so are there any advantages/ disadvantages of doing research on a grant or as an intern when applying to grad school?

I did western blot with a standard, and a protein sample. I got a band for my standard and many other “unspecific binding” bands, however, i did not get any band for my target protein. What could be the reason ?

I just got my exam back, i got a B+, i know its good however compared to the average of the class its bad. The mistake i made that got me here was this question “how do cells ensure the accuracy and specificity of cargo delivery within the complex network of vesicular trafficking pathways?” I explained in details the specificity and accuracy of SNAREs however i did not mention the coat proteins because i felt like they were irrelevant to the question that asked about the SPECIFICITY and ACCURACY of the vesicular transport, it turns out i had to mention them and only got half the grade on that exercise (which was about 30% of the total grade). My question is was it unfair for my professor to remove so many points over not mentioning such an irrelevant detail or are coat proteins actually involved in SPECIFICITY, thank you.

I am studying chemistry and botany in college so I genuinely am curious about the validity of these claims and the specific mechanisms behind this if anyone knows:

Certain plants contain notable amounts of plant based hormones called phytoandrogens, which are structurally similar to testosterone or estrogen. Just from Google it seems like pine species contain testosterone. Similarly, soy beans contain estrogen.

I’ve heard of gym bros refusing to eat soy beans because they claim it will mess with their hormones due to the presence of plant hormones resembling estrogen.

Is this true?

Do phytoandrogens actually have a significant effect on human hormones? Or is it just bodybuilding gym bro hogwash?

Please let me know any biochemical insight you have!

Question from a new undergrad student here. All elementary biology textbooks seem to treat the fact that RNA is single stranded as a fact, but I was wondering what is keeping RNA from associating with it self to form a double helix like DNA?

I understand the fact the RNA in cells are transcribed one at a time so it’s pretty much impossible for dsRNA to occur naturally, but what about in labs? If we have a vial full of RNA will it naturally keep to a ss form or will it form a ds structure like DNA?

I’m working on an ecology project where we will be using owl pellets as bio indicators for heavy metals. There are some studies on this but most of them use a microwave digester (which I don’t have access to) so I was thinking maybe I can get away with using a sonicator with hot water instead of the microwave digester. If anyone has any advice or info on something like this please let me know.

Or if your knowledgable about ICP-MS technology I would love to talk about it and ask questions. Any help would be greatly appreciated.

My study is in Egypt and the curriculum in our college are very outdated and lack many essential topics. What are the fundamentals that would be embarrassing for a biochemistry graduate not to know?

Hi, everyone. My lab just purchased a LEX48 reactor system and I was wondering if anyone has any experience using one and what they thought of it, and or had some useful tips and or tricks?

I had a biology class today(a level) she showed us some metabolic pathways(although those weren’t important) and I just need some conformation that this is beta testing oxidation.

Trying to decide what classes to take?

Want to know what the job outlook is with a biochemistry degree?

Trying to figure out where to go for graduate school, or where to get started?

Ask those questions here.

Good morning, I'm an American PhD. student in a Biochemistry and Microbiology department. The lab I currently work for is a yeast lab where I study Prions and Prion adjacent proteins using primarily microscopy but I have acquired skills in cloning and purification as well. I still have a little bit to go before I graudate but I'm over the halfway mark and I am starting to make plans for work afterwards. I know I'm more interested in industry over academic work and something like a Applications Scientist sounds nice to me. Hell I'll even work in sales over having to write grants. I don't want to limit my job search to strictly America even if it can be tough to move overseas, but I wanted to ask. Where might there be opportunities for me outside of the US, and do you have any advice for me when it comes to that?

I am interested in locations that have good work opportunities as well as may be easier to move to in regards of ease of the process.

Im looking for an proper application note for an HPLC (Agilent 1260, with DAD) to analyze steroides (f.e. E1,E2,EE2,E3) in waste water.

No GC-MS or UV available.

Does anyone has some great papers? Actually i cant figure out how it works

I'm a current Junior studying biochem on a F1 visa and almsot every summer research/intership program requires US citizenship/residence, and I'm wasting hours even looking for jobs, not even applying.

Are there websites/filters that show me eligible jobs/any helpful tips on where to continue looking?

Hey guys doing my a levels and have applied for biochem at uni and god good offers. I am having second thoughts though because I feel like it will be a really hard degree. My moms friends daughter did biochem and got 5 As at a level and she refused to do a masters even when the uni offered to pay for it cos she said it was too hard. I’m so scared. My grades are okay I’m predicted A psych A* bio and C in chemistry so I feel like that’s not great right. I just memorise for bio and psych but with chem I find the problem solving and maths harder which is what the who degree is right? I’m also worried about the job market as it’s often not very positive on here. I was thinking of switching to psychology as the pay is better but I’m not a huge people person. Point is do u guys think I’d be okay doing biochem if I work hard? And is it easy to get a job in a random office job or something if I don’t like it? Also do you often need a masters to get okay pay because if you do I might switch to psychology cos it’s the same money but I’m gonna earn more for psychology. I’m genuinely so scared and it’s really affecting my mental health at this point. EDIT: thanks so much for the replies guys you’ve all been really helpful xxxxxxx

Hey everyone,

I’m an exercise physiologist and I’ve been learning about the energy systems forever. Always loved ATP synthase and I want to get a tattoo of it. Could anyone please verify if this drawing of it is “accurate”? I want no colours just black ink.

Appreciate your help!

I graduated with bachelors in biochemistry couple months ago, and I was lucky enough to be hired as an R&D scientist, specializing in endocrine immunoassay development. It is a small startup at the moment, but considering its financial stability and popularity in the field it has a promising trend.

Now the question is, should I stay in industry or go to grad school? I’ll clarify first that I don’t have a particular topic that I love at the moment, and I don’t mind either options. I see virtually 95% of my friends going into masters and PhD, saying they want better opportunities in the future. But I also hear from some people saying that work experience is more valuable than academic degrees if I intend to end up in industry.

It seems like half is telling me that having bachelors only will give me an upper cap in potential income soon, while the other half is telling me that I’m in the right path.

For maximizing income in the end, what is the most reasonable choice? Would masters/PhD be an absolute necessity if I want to eventually move up the ladder and make 6 figures (or not, just for maximum potential)?

Apparently, I'm just supposed to go find one. Not really sure where to look, so I thought I'd try here! I have to get a few fillings done. I'm a breastfeeding mom and hope to be pregnant in the next year, so I try to eliminate whatever toxins I can. Admira Fusion is the filling material my dentist uses (photo included). I'm concerned, because I see that there are nano-particles in it. I know that nano-particles can cross the blood-brain barrier. Would nano-particles be released into the body from this filling material?Maybe not, because fillings are cured?

I have the option of ordering Activa brand composite filling instead. Would that be safer for breastfeeding/pregnancy?

And finally, does lidocaine pass through breastmilk? If so, how long would I need to pause nursing after getting my cavities filled?

Is it worth it when pair with Computer science? What some course requirements to take it ?

Hello! :)

I'm looking for a mobile phase for TLC that I can use to visualize sugars like glucose and fructose. Does anyone have any suggestions?

I'm a college senior who's going to graduate in the spring with a biochemistry major and a business minor. For the last year or 2, I've been pretty set on going into pharmaceutical sales, healthcare/ life science consulting or something similar. I had a summer internship in biotech sales at a large agriculture based company, 2-3 years of experience working in academic research labs and pretty good grades with a 3.70. I really want to get a position at a large pharma, biotech or consulting firm, but it seems like applying is almost a waste of time as every "entry level position prefers 1-3 years of sales or consulting experience and I keep getting denied. Anyone have advice on how to break into one of these roles? I know networking is important, but messaging people on LinkedIn hasn't been a ton of help either. Thanks!

Hello! Quick question- I understand that NAD(P)+ is an allosteric activator of glucose 6 phosphate dehydrogenase which helps to regulate the Penrose phosphate pathway, but does NAD(P)H directly inhibit it also? Do both of these happen in parallel to negatively regulate the enzyme, or is it just one or the other? Thanks!

I'm studying the Michaelis-Menten equation, but there are some concepts I don’t quite understand. Basically, I understand that as the reaction progresses, the substrate concentration should decrease because it binds to the enzyme and is therefore consumed. So, hypothetically, at the beginning of the reaction, we have a maximum substrate concentration that gradually decreases. However, I’m not sure whether the initial velocity, V0, decreases as the reaction progresses or increases. It would be incorrect to say it decreases; it would be more accurate to say it increases. Initially, it increases linearly, but as the reaction continues, it increases less intensely until it reaches a plateau, which will represent the theoretical maximum velocity.

Hi, I've been studying the regulation of TAG synthesis by insulin, and I do understand why insulin stimulates the production of TAGs. But I don't see clearly why people with insensitivity or lack of insulin causes most of the Acetyl-CoA to be used to produce Ketone bodies. Can you explain to me briefly why people with diabetes have this increased production of ketone bodies?

Alright, this is a long shot here but I am interested in recreating this peptide sADP5. Other peptides like P11-4 are generally available but this one is not. Again, I'm interested in obtaining it, but I am way out of my depth.

These two references are as close as I have come to specific details. Is this enough information or do I need to keep digging?

https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c01039 (Same thing different site https://pubmed.ncbi.nlm.nih.gov/36854046/ )

https://patents.google.com/patent/WO2017123986A1/en

I'd also appreciate a good place to start learning about peptide synthesis so I don't feel so out of depth.