Hey guys, I'm here to discuss mycellium lifespan. I have used a syringe of Makilla Gorilla (MG) and have been successfully growing several monotubs. To speed up the process instead of doing syringe to grain inoculation I have been doing g2g (grain to grain) transfers. And I noticed that after some time it looks like it takes longer and longer for jars to colonize, and some of them don't even finish colonizing at all. I do understand that it might be some bacteria, but let's not go that way for now. I do work under flowhood with an h14 filter and my sterile tequniques are up to par. The question is does mycellium really get tired? Does it have like some kind of genetic lifespan. What kind of experience have you had? I'm labeling my generations like this. So if I have an LC (liquid culture) and I make a jar from it, that jar will be gen1. If I transfer from that first jar to let's say 10 jars, they will be gen2. So have far do YOU go on your transfers? Also does it matter how big is your transfer amount? I use a full table spoon with top if possible. I tried 2 spoons but then difference in speed is only like 1-2 days so I prefer to inoculate more usually. Does anyone understand the genetics deeper? Is there a certain amount of weight each piece from petri dish can produce? Or like a certain length in miles it can grow into? Or maybe there is time involved?

So now, when I do g2g transfers and I am too far out like gen 5-6 I have to reset. This strain does not give spores remember. So I have 2 choices:

1. I use existing LC or
2. I take the sample of one of the best specimen (fastest, clustering, biggest), I clone it on to the petri, make an LC from petri and then use that LC.

Thoughts and tequniques would be appreciated. Let's share some knowledge about the generations and genetics.

I was thinking about making a heatbomb to place on bottom section of my closet.

I just added my grain spawn to the bulk substrate two days ago and I'm already seeing fast growth. How can I tell when I should introduce fruiting conditions? Additional information: 66qt monotub, roughly 3 gallons of coco coir, 2 gallons vermiculite, 2-2.5 gallons of colonized brown rice. (first two pics are from two days ago when the spawn was added and the last three are from today)

https://preview.redd.it/77d523e0645e1.jpg?width=3024&format=pjpg&auto=webp&s=32f7e7ced439d254cea21a3bfab18510a71be4ab

https://preview.redd.it/6hr3v3e0645e1.jpg?width=3024&format=pjpg&auto=webp&s=9bda49d1bacf21684109e6650efb629091e13582

https://preview.redd.it/pn2f24e0645e1.jpg?width=4032&format=pjpg&auto=webp&s=a9b4fea18a5cf38433ae1f12432d02a1014442bb

https://preview.redd.it/70ru33e0645e1.jpg?width=3024&format=pjpg&auto=webp&s=278a49f0e3db73355411248962db4d8cc7ec1356

https://preview.redd.it/r017i3e0645e1.jpg?width=3024&format=pjpg&auto=webp&s=d9bbcb7984c2b34da7d8e5d3e346691ebb99b7b9

I dunked and now there is this yellow brown shit, should I toss this ?

Long story short my heat source stopped working. So my tubs were in colonizing stage for 3 days without heat. Temps stayed above 60. Well now that I have reintroduced heat one of the tubs has started pinning.. they have been colonizing for 9 days. Shoebox tub.. do I need to induce fruiting or try to colonize more?

Is manure used only for microbiological purposes as in beneficials? Or is manure used for nutritional and enzymatic purposes? I have access to a shit load of salmon food, which I’m thinking about doing an experiment and adding some of it to substrate, but at the same time if people already know that dog food ruins substrate colonization that I’m not gonna do it, does anyone have any insights into this? Thank you.

Any help or guidance is greatly appreciated. I’m very new to the hobby

hi what's up I'm interested in growing magic mushrooms, do you know where I can order them encrypted, because it's illegal in my country?

I read that golden teacher likes warmer fruiting conditions like 20C above

I prefer cold. It’s around to 10C to maybe 15C What can I do to get them fackers fruting I’m kinda devastated. I figured out how to work steril just to get fucked by temperature 🥲

I wanted to not have to buy more electrics.

• I can’t keep the humidity at 80-90. it’s at 65-70 and I don’t know why it’s not getting Higher.

Title asks the question. If humans must consume food to reproduce etc and fungus aids that, is there a discussion about wether over a long enough time span fungus kinda farms humans?

Do I need to throw this? :(

Been troubleshooting this kit for a couple days now due to stalled growth..

But not sure whether I need to just throw it now and class it as a First grow attempt failure.

Gutted…

Back to research & back to the drawing board it is!

Any thoughts greatly appreciated!

Ok soooo I just at what I think was a "vic" of shrooms (idk the strain) and I had eaten like 8 mozzarella sticks before hand am I gonna trip still or no it's been like an hour and Idek if I got real shrooms tbh

I’m going to walk with the stars tonight. I don’t know if there are any peaceful friends here who would join me. I would very much enjoy the company. Human to human connection. ❤️❤️❤️

Hi! Getting serious about this and setting up my growing areas in a large 5x10 closet (no windows). I was going to clean the area, set up some metal shelves - and that’s as far as I’ve gotten. The closet is huge, more like an attic space, so I’m using only one end of it and the other is storage. It has a regular door, not bifold or anything.

My questions:

1. Do I need a separate grow area for the colonization of the grain vs. S2B/fruiting? Do I want to keep that separate?

2. If yes, should I divide it up into different areas with plastic sheeting? Or would it be better to just get shelving with zip up covers and keep in all in the same section?

3. Does any of the above sound like it’s going to limit airflow too much?

If they’re in the same area, I wouldn’t be able to keep temps different so maybe that answers my question.

Any advice or ideas would be great! I’m not going to worry about air pressure right now, this is more than enough.

I have an SAB and will be using that to inoculate.

Well after solid pins were formed and the QTY of the overall flush was apparent, I found a patch of Trich on a far side of the substrate that wasn't fruiting (in a mono tub). I sliced it out and carried on, as the harvest on the other side of the tub was looking great. By the time I harvested, I had enough to fill my 5-tier dehydrator twice, but then did not attempt a second fruit as the trich area was getting larger and larger. So, I ditched the cake and scrubbed everything down, and will repeat another hundred times.

So here finally is the question... my harvest is still ok, right? Could the Trich spores in my grow space still affect them as they dehydrate? Will they, uh... be safe for further examination?

This group has silently kept me in the fight. I feel like I’m breaking. I thought I was moving on and looking. Then I sink. Straight back to my hole. Divorce is painful. Even when you’re both on the same page that it has to end. Living life alone is hard and lonely. Here I am again pushing people away. The few people who will actually listen, I’m pushing away. As I’ve done so many times. I was going to go walk with the stars tonight but just felt unsafe. I’m not asking for help from anybody, i just needed to tell you that I’m struggling. Peace seems like it’s at the end of the rainbow. It’s simply not there. Love you all.