/r/microscopy

Photograph via snooOG

In science class, you always wished you could play with the microscope a little bit longer. Now that you are an adult, you actually can. Cooler and with more bang for your buck than r/telescopes, microscopy lets you do real science!

Microscopes and the the Microcosmos

Community for identifying and discussing microphotographs as well as microscopy techniques and microbial life in general. Researcher or microscope owner with some cool pics? Share it with us! Saw a weird critter in your pond samples the other day? Show us here and maybe we can sort out what it is!


Rules

  1. All media posts must include a description of the setup used to capture the subject. The details can be included in any combination of the title/post/or comments. At a minimum, the objective magnification, scope model, camera, and sample type are required. Additional info is encouraged, such as the NA of the objective, the illumination technique, how you prepped the sample, etc.

2.Images should be cropped and of a high standard

3.No offensive language or toxic behavior

4.Request permission before self promotion

5.Request permission before posting medically significant questions Medical professionals, researchers, or students are able to request permission to post medical questions.

6.Human samples must be marked NSFW with the title clearly indicating what the sample is

7.Human samples must be not include personal information on the identity of the sample source. If you post a human sample, you can not include the name or other irrelevant personal information of the person the sample came from. If you post an image of a human sample that is from your own person, you should not indicate that it is from you.

8.No reposts or uncredited plagiarism Reposts are not allowed, but . This sub is for original content. However, you may post an image from another person, if the image has not previously been posted, and if you credit the origin of the content with a link to the content owned by the creator e.g. a link to the image on the creator's instagram

9.No purposeful misinformation


Note: This is a public subreddit, so please avoid posting unpublished data unless you wouldn't mind it being distributed far and wide.


Microbiology r/microbiology/

Electron Microscopy r/electronmicroscopy/

Protists (~microbial eukaryote) r/protist/

Macro Photography r/macro/

Journey To The Microcosmos YouTube

Microbe Hunter YouTube

/r/microscopy

55,345 Subscribers

8

Lunch time

BRESSER Science TRM 301, recorded with Canon 600D, 100x and 400x

No idea what it is 😜. I'm 2 weeks old in terms of hobby age.

4 Comments
2024/10/31
21:15 UTC

6

Tiny flagellate digging for food - 1000x

2 Comments
2024/10/31
21:09 UTC

45

Tardigrade among algae

Omax binocular scope with blue Erenberg filter at 200x recorded with iPhone 11

2 Comments
2024/10/31
16:57 UTC

2

Plasmolyzed red onion cells

1 Comment
2024/10/31
15:31 UTC

3

Microscope recommendation for my wife's birthday?

Hello! My wife is somewhat obsessed with microscopy and has an antique low magnification scope that she uses, but its very janky and hard for her to take photos with it. I'm looking to get her a better microscope for her birthday, but I know nothing about them. I've asked her for guidance but she's just beginning and isn't sure what to buy.

I'm hoping for something comparable to the Journey to Microcosmos scopes that they were selling for a while. Preferably in the $400 range? If we could get additional hardware in the future to hook up her DSLRs that would be a huge benefit.

Any advice would be greatly appreciated!

5 Comments
2024/10/31
15:10 UTC

3

Help

I've been working on imaging some small rodshaped bacteria at x100 using a confocal microscope with a photomultiplier tube. They're pretty small, about 0.5 um x 2 um. I've tagged two proteins with fluorescent tags (EGFP and mcherry) by translational fusion. I usually do live microscopy, an image every 2.5 mins for like 6 hours to watch them divide.

Currently my time is limited because I mount my cells on an agar plug. This traps them between the cover slip and the plug to stop them from moving, it works really nicely and the plug is made from media so they have nutrients to survive. The only issue is the plug dries out and after about 5 hours, they die because the plug is dry. I've considered trying to seal it but it's extremely difficult as capillary action just pulls nail varnish under the slip. I'm also worried about oxygen as it's a long time and sealing may prevent diffusion. Does anyone have any suggestions on a better method I could try?

2 Comments
2024/10/31
04:29 UTC

4

What to buy as second microscope

I recently bought a microscope that can go up to 1000x and I’ve enjoyed it a lot. There are some flaws with it like the light gets dark even at the max brightness when I go up to 1000x and the image becomes very blurry at 1000x. I also see microorganisms in water that are way to small to see clearly on 1000x. I would like to get something maybe like 1500-2000x at a 300$ and below range. Any recommendations?

8 Comments
2024/10/31
00:45 UTC

5

Ciliate division at 100X

Hutact Beginner Microscope. They divided as soon as I started recording so I still was trying to stabilize my hand ( The phone apparatus of the microscope is really useless so I have to use my hand to record it) but I thought it is still worth to see.

1 Comment
2024/10/30
19:03 UTC

90

Microbes Everywhere

8 Comments
2024/10/30
18:14 UTC

18

Nauplius Larva Poops - 400x

2 Comments
2024/10/30
14:47 UTC

4

Aeolosomas, Paramecia, Copepods & Vorticellæ (Magnification 100 & 200x) from my aquarium

1 Comment
2024/10/30
10:00 UTC

8

Stentor (magnification 400x) from moss [Toronto, ON, Canada]

1 Comment
2024/10/30
09:56 UTC

78

Gastrotrich

6 Comments
2024/10/30
01:40 UTC

47

Algae?

14 Comments
2024/10/30
01:07 UTC

2

Adapter to attach Barlow lens to objective without threads

Hi all, I recently got a Bausch and Lomb Academic Stereo Microscope (like this) to use for amateur/hobby watch repair. The field of view is a bit small for my use, so I'd like to get a 0.7x Barlow lens for the objective - my issue is that on this model, the objective lens is not threaded on the inside - so there isn't an easy way to attach a Barlow lens. (Edit to say: it's more like this scope doesn't have an objective 'lens' - it's just the lower part stereo head isn't threaded at the opening)

I've been looking for some kind of adapter that I could attach around the objective lens (using set screws likely) that would have interior threads that I could screw on the Barlow lens to that. Maybe this wouldn't be possible because that would change the distance between the objective and the barlow lenses making focusing impossible? But wouldn't that mean every scope on the market would have to have the exact same distance between objective lens and the threads to ensure all barlow lenses would be compatible?

If anyone has any ideas, I'm open to just about anything. I just can't seem to hit the right keywords in my search to bring up anything useful.

2 Comments
2024/10/29
22:08 UTC

2

Good microscope for 200€?

Which microscope is recommandable for 200€?

0 Comments
2024/10/29
21:54 UTC

3

Question for the community

Hey everyone, I hope it's ok to post this question here. I'm a creative who knows nothing about microscopes but want to take some photos of something specific under a microscope (the subject is a common food item) for a marketing / branding exercise. i am guessing it would take no more than a few hours.

i haven't used a microscope since grade school and don't have any friends who are doctors/scientists, so i am at a loss on where to begin my search to find a microscope that i could use for a few hours. any suggestions on how to go about finding such a thing? i looked into renting one, but i worry that i might not be able to use it without assistance (i could be wrong), and because the city i live in is small i couldn't find any suppliers who rent scientific equipment. there are a few universities in town with medical schools, but i'm wary of reaching out to them and sounding ridiculous, since i don't know the first thing about laboratories or microscopes :D

thanks in advance for any advice! i love what you guys are doing here, your photos and videos are beautiful and very interesting!

7 Comments
2024/10/29
18:23 UTC

404

Plant cells at 1000x with immersion oil

26 Comments
2024/10/29
15:17 UTC

39

Rotifer exploring red algae.

Sample taken from my bird bath. 40x and 60x zoom.

1 Comment
2024/10/29
14:41 UTC

2

The Euromex Observer plan achromats resale?

Hi. I would really appreciate some help here. I'm considering purchasing the Observer and I would need to sell the standard plan achromat objectives when I decide to upgrade, but I have no experience and it's really hard to me to judge if this willbe at all possible. Please, can you explain this to me? How can I determine if there will be potential buyers? I suppose this includes things like parfocality and corrections but I just feel overwhelmed by the issue.

1 Comment
2024/10/29
13:22 UTC

1

Sony α6400 vs Canon EOS R50

Hi everyone, I'm thinking about changing from my Canon EOS 600d DSLR to mirrorless camera. Mainly because my current one is pretty old, clunky and video recording is rather bad quality. Plus I have problems with all the attachments to my microscope. Since it has a pretty high flange distance and type of build that limits possibilities of connecting it to my stereo microscope (Bresser Analyth STR Trino).

It works "ok" with my compound micro, but I have to literally put the camera with naked sensor on the phototube as any other adapter would raise it up and I wouldn't be able to reach focus again.

I'm considering those two mirrorless cameras

Sony α6400 vs Canon EOS R50

  1. Both are similar video and picture quality
  2. Canon has advantage in videoes (both 4k and FHD) as it doesn't introduce additional crop, while Sony does quite significant for 4k (1.22 at 30 FPS) and slight for FHD (1.14 at 120 FPS). This plus the sensor size can make the final picture quite severly zoomed it
  3. Sony can be charged via usb cable at the same time when used with PC, while Canon doesn't allow that (only with dummy battery which I have 2 now and their performance is mediocre)
  4. Canon software to control camera is crap, really it's really bad, while I hear that Sony software is pretty decend.
  5. Price is almost the same with small advantage towards Canon.
  6. Canon R mount T2 rings seems to be hard to find :/

I struggle because I hear that Sony's sensor is really good in low light conditions and I see that a lot of fellow photmicrographers are using Sony cameras, however the crop factor on FHD. I'm also inclided towards the ability to charge the camera while having it connected to PC, as I want to observe mostly on my monitor with ability to record for longer time.

While no-crop factor on Canon is a pretty good advantage.

The software is the least of an issue, as I believe there has to be something good (that doesn't introduce lag when watching LV), but any recommendations are welcome.

Any experience with any or both of the cameras? Suggestions are welcome.

Thanks in advance!

2 Comments
2024/10/29
09:33 UTC

3

Finding iphone adapter for 23mm eyepiece?

Hi everyone,

I am currently looking at a Amscope B-120 which according to their website has a Ocular diameter of 23mm. However I haven't been able to find an iPhone adapter for eyepieces this small. Am I looking at the wrong measurement? All of the adapter I have seen have been at least for 23.2 mm all the way up to 28 mm.

Any ideas?

thanks.

1 Comment
2024/10/29
04:16 UTC

10

help with ID??

400x total mag, Amscope b120c, iphone camera, freshwater sample from planted aquarium.

3 Comments
2024/10/29
03:19 UTC

15

Life among death

Thought people might enjoy this video I got some months ago.

3 Comments
2024/10/29
00:25 UTC

0

Cigarettes residue after blowing onto a slide. Sorry about the quality. 800 magnitude celesteon 2000x

I hope this doesn’t offend anyone. Next slide is like to blow vape on a slide to see for myself what the differences are.

3 Comments
2024/10/28
23:55 UTC

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