/r/microscopy

Photograph via snooOG

In science class, you always wished you could play with the microscope a little bit longer. Now that you are an adult, you actually can. Cooler and with more bang for your buck than r/telescopes, microscopy lets you do real science!

Microscopes and the the Microcosmos

Community for identifying and discussing microphotographs as well as microscopy techniques and microbial life in general. Researcher or microscope owner with some cool pics? Share it with us! Saw a weird critter in your pond samples the other day? Show us here and maybe we can sort out what it is!


Rules

  1. All media posts must include a description of the setup used to capture the subject. The details can be included in any combination of the title/post/or comments. At a minimum, the objective magnification, scope model, camera, and sample type are required. Additional info is encouraged, such as the NA of the objective, the illumination technique, how you prepped the sample, etc.

2.Images should be cropped and of a high standard

3.No offensive language or toxic behavior

4.Request permission before self promotion

5.Request permission before posting medically significant questions Medical professionals, researchers, or students are able to request permission to post medical questions.

6.Human samples must be marked NSFW with the title clearly indicating what the sample is

7.Human samples must be not include personal information on the identity of the sample source. If you post a human sample, you can not include the name or other irrelevant personal information of the person the sample came from. If you post an image of a human sample that is from your own person, you should not indicate that it is from you.

8.No reposts or uncredited plagiarism Reposts are not allowed, but . This sub is for original content. However, you may post an image from another person, if the image has not previously been posted, and if you credit the origin of the content with a link to the content owned by the creator e.g. a link to the image on the creator's instagram

9.No purposeful misinformation


Note: This is a public subreddit, so please avoid posting unpublished data unless you wouldn't mind it being distributed far and wide.


Microbiology r/microbiology/

Electron Microscopy r/electronmicroscopy/

Protists (~microbial eukaryote) r/protist/

Macro Photography r/macro/

Journey To The Microcosmos YouTube

Microbe Hunter YouTube

/r/microscopy

57,269 Subscribers

1

What microscope to buy for a budget of up to 530€ max?

I'm a biology student and after using my ok childhood microscope for years, I'd like to buy a better quality one for personal use.

Do you have recommendations? Whats the best one for this price in your opinion?

I am searching for a compound microscope, ideally trinocular, with 100x oil immersion, achromatic, etc. Ideally with köhler illumination, but I guess it's rather rare in this price level.

Also it would be great if it would be at least updatable to darkfield and polarization microscopy. I guess it's almost impossible to find a microscope that is already equipped with these features in such a low price level.

I mean, I found this one which sees to be equipped for darkfield microscopy https://amscope.com/products/c-t490-dk?srsltid=AfmBOop-lX8E7CfblMeHHcHSiYzBtqzQi7Ou4xLQXXmEqeYs_97lUPo- Is this worth ir? Or should I rather buy an updatable high quality brightfield microscope?

Sorry for mistakes, english isn't my native tongue.

0 Comments
2024/12/01
18:37 UTC

0

Seeking Input: What Are the Unresolved Challenges in Digital Microscopy Systems?

Hi everyone,

I’m conducting research to better understand user experiences and challenges in the world of digital microscopy. With advanced systems like the Keyence VHX-X1, Zeiss SmartZoom 5, and Olympus DSX1000, the market offers powerful tools designed to streamline imaging and measurement workflows.

However, I’m curious if there are specific pain points or unmet needs in these systems that users are experiencing.

Here are a few aspects I’d like to explore:

  • Optical Quality: Are the resolution, clarity, and imaging capabilities sufficient for your needs? Do you encounter limitations with magnification or depth-of-field in practical applications?
  • Software Features: How intuitive and powerful is the software? Are there frustrations with features like measurement tools, 3D visualization, or reporting? Is there a lack of integration with other software or systems?
  • Ease of Use: Are these systems user-friendly enough for both beginners and experienced users? Are there any workflow bottlenecks?
  • Cost vs. Value: Do these high-end systems justify their price, or are there areas where they under-deliver for the investment?
  • Reliability and Maintenance: Are there recurring issues with hardware durability, software bugs, or challenges in getting timely support?
  • Specialized Applications: Do you find these systems versatile enough, or do they fall short in specific niche applications (e.g., in electronics, automotive, or materials science)?
  • Performance vs. Marketing Claims: Have you experienced gaps between the advertised capabilities and real-world performance?

I’m particularly interested in how these challenges manifest in industrial and manufacturing applications, where digital microscopy plays a crucial role.

Your input—whether from hands-on experience, industry trends you’ve observed, or general impressions—would be invaluable. If you’ve encountered problems or have ideas for improvement, I’d love to hear them.

Feel free to share your thoughts or recommend resources that could help shed light on this topic. Let’s start a conversation about what’s next for digital microscopy!

Thanks in advance for your insights!

0 Comments
2024/12/01
18:20 UTC

2

Slide and cover slip

Hello everyone, I would like to ask if there is any object that can be used to maintain the distance between the slide and the cover slip under a microscope. This is because when I observe protozoa, they often get distorted if I place these two pieces of glass directly on top of each other. Thank you all.

4 Comments
2024/12/01
14:32 UTC

2 Comments
2024/12/01
13:06 UTC

1

Opticon BMLU Bioptical Electric Laboratory Microscope

I was gifted this vintage microscope. I’ve been looking for a microscope to look at cells, small protozoa, and all kinds of fun stuff (that unused Biology degree may finally be used!). I can’t find much info about this model. I’m wondering if it is a good platform that can be improved upon with new lenses etc. and can’t find much information about it. It has 4x, 40x, 60x magnification so, with the eyepieces, 40/400/600 mag.

If anyone is familiar with this model and can give me some advice, it would be greatly appreciated. I’d rather spend the money on improving this scope than on buying a new one, if possible. Thanks in advance.

1 Comment
2024/11/30
23:29 UTC

1

Coverslip Stains?

After staining a blood or cerumen sample with Diff-Quik, rinsing, and drying, is it advisable to apply a coverslip when using the 100x oil immersion objective?

Would the coverslip help prevent any residual staining solution from mixing with the oil and potentially contaminating the objective lens, or is this not a concern given that the sample was rinsed and dried?

Additionally, would using a coverslip help stabilize the sample and prevent any displacement of microscopic findings when adjusting the stage during observation at 100x oil magnification?

9 Comments
2024/11/30
21:37 UTC

0

C-mount?

I have a Bresser Researcher Trino. I bought an adapter since I already have a camera with C-mount size? But that is useless. I don't even know what it is. The scope came with some weird tubes. But nothing fits? Camera looks like this. How do I fit it?

https://preview.redd.it/iawndohzt34e1.jpg?width=800&format=pjpg&auto=webp&s=b13dfb5c852ef7bd5e4587237af9ad67d8743423

0 Comments
2024/11/30
21:12 UTC

3

What is a good microscope for around $400-$800

I am sort of experienced with microscopes and I’ve used them a lot before, but this is my first time actually buying one, I was looking at the one on micro safari for 799$ currently, but I was wandering if anyone has some good suggestions for microscopes, I want to use it for looking at cells, blood, possibly dna, and bacteria or creatures!

10 Comments
2024/11/30
20:00 UTC

1

What digital microscope should I get? Preferably $100-200.

First time, but I want something that is quite powerful and will last a while.

0 Comments
2024/11/30
16:36 UTC

6

Mom looking for Microscope for son- please help.

I have been reading up on microscopes, but have limited knowledge on what might be a best option.

What I do know:

  • Trinocular
  • Camera compatible

About him and use:

  • He asked for- Swift SW380T 40X-2500X Magnification
  • He is 18
  • Looking to get into a biology field for profession
  • Currently doing for hobby/research/learning

Upon research, the one he asked for looks decent to me, but I feel he is being modest with what he is asking for. I would like to purchase him a good quality microscope that will last him a while and provide him nicely detailed views of what he will look at. If the Swift can do that- fantastic- but a little hesitant because I know a quality instrument runs thousands. I would appreciate any help with pinpointing a good one, any experiences with different brands, etc. I would love to learn so I can help support and have conversations with him on this journey.

Budget: $1,500

TIA!

8 Comments
2024/11/30
16:12 UTC

65

Marine Flatworm

Scope: Motic BA310 / Mag Objective: 4x(40x) / Camera: GalaxyS21 / Water Sample: Biscayne Bay

17 Comments
2024/11/30
09:20 UTC

7

Experiments with basic darkfield

3 Comments
2024/11/30
06:23 UTC

3

Got some type of mite and contamination on a petri dish... anyone can identify either/or?

Sorry new to microscopy and took pic on phone

3 Comments
2024/11/30
02:58 UTC

16

What is this guy?

I just started playing around with my first microscope and came across this guy in some old water I had plant cuttings sitting in. Any idea what it could be?

5 Comments
2024/11/30
00:34 UTC

69

Human lice, they're adorable for a parasite

14 Comments
2024/11/29
21:43 UTC

2

Which microscope should I get?

Swift SW380T or Amscope B120c? I have the budget for both. I'm just wondering which one is better. They have the same magnification.

3 Comments
2024/11/29
17:05 UTC

1

I’m new to microscopy, just got mine 2 days ago! Any tips for a beginner?

I have the National Geographic 40x-1024x microscope if that means anything. Just general knowledge you think a beginner could have use of would be greatly appreciated!

2 Comments
2024/11/29
15:43 UTC

10

Dark Field Question

Hello, i am a physics student helping at a radiation lab where there is a microscope used for studying behaviour of cells under radiation. Im a total noob at microscopy and i took this picture last week while changing things at random in the microscope and didnt think much of it. Now reaserching more i think it might be able to take those cool images with a dark background, even though the one i took doesnt look like the ones im talking about. I won't be able to see it again untill next week, but i was curious, can anyone tell if this is dark field or not, and what im doing different?

The slide is of a plant stem, 10 x 40 if i remember correctly, i dont know the model of the microscope or the camera model, this was just a test and i dont have the info right now.

3 Comments
2024/11/29
15:31 UTC

5

What do I need to see these guys? (text in comment)

4 Comments
2024/11/29
13:40 UTC

33

A loving Monocyte

5 Comments
2024/11/29
07:48 UTC

2

I got a Bioscope model 60-A for my birthday.

Is there an online manual for them? It would be cool to see if it would work again and how to properly use it. It comes out of the case upside down so I'm not sure how to set it up. And if anyone has any cool facts or advice let me know. And replacement parts.

4 Comments
2024/11/29
03:27 UTC

1

I got a Bioscope model 60-A for my birthday.

Is there an online manual for them? It would be cool to see if it would work again and how to properly use it. It comes out of the case upside down so I'm not sure how to set it up. And if anyone has any cool facts or advice let me know. And replacement parts.

0 Comments
2024/11/29
03:20 UTC

3

Need help ID-ing this teardrop-shaped microbe

Hi everyone! I’ve recently collected some river water and cultured the microbes growing in it. I can’t seem to find this one among the common freshwater microbes describes online. There’s loads of these dark, teardrop-shaped microbes that seem to rotate while they move. They don’t seem to have a flagellum and the round end seems to be where their mouth is because food seems to get sucked into there. I was hoping to get a lot of paramecia and rotifers but these guys are cool too!

5 Comments
2024/11/29
02:12 UTC

1

Anyone have any experience converting between Leica and Zeiss image files?

I have some .lif image files that I got from my Leica confocal fluorescent microscope and I'm trying to import them into Zeiss Zen Blue so I can export them as .czi files. The reason being I want to use an automated toolkit to analyze my images for me since the manual process is quite time consuming but the toolkit only recognizes .czi files. Anyone have any experience with converting files between the two brands?

7 Comments
2024/11/28
22:00 UTC

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