Hello!

I recently learned about suboxone and methadone in my clinical medicine class, but still feel like I don’t have a great grasp on it. Can someone please tell me the difference between suboxone and methadone besides suboxone having a ceiling effect and being a partial agonist while methadone is a full agonist, and that methadone is administered by an opioid treatment center while suboxone is prescribed? Also are both of these drugs forbidden to be taken by individuals with certain occupations, ie pilots? Any additional info you think would be helpful is greatly appreciated!

Thank you!

Hi I just started a new course in pharmacology but I realized I lack a lot of pre-requisite knowledge for calculations and I’m stuck on converting DPM to mol. I’ve attempted it but don’t think I’ve done it correctly yet. I first took the DPM and divided it by 2.22 x 10^12 to give me the value in Ci. Then to gain moles from the Ci I divided the Ci value by the specific activity to give me the value in mmol. Is this correct? Thank you :)

Pharmacology student here. I have been giving this data set from a saturation ligand binding study and need to produce a scatchard plot. Can anyone explain to me why the first row of data reads 0 and if I am okay to plot this but essentially ignore it when drawing a line of best fit.

The reason I ask is if I ignore the 0 when creating the straight line it almost perfectly matches my Kd and Bmax values for the nonlinear curve graph I’ve already done. But if I include the 0 it distorts it quite heavily and it is wildly different.

If I can’t ignore the 0 can someone give me an explanation I can write as to why the values from the 2 different graphs differ?

Thanks!!

Hello! I'm a first year medical student and have a pharmacodynamics-related question. In the context of non-competitive antagonism, what happens to the value of EC50 in the presence or absence of spare receptors? Some resources say it remains unchanged, while others say it will either increase or decrease. My lecture slides say that EC50 will increase in both the presence and absence of spare receptors but that does not make any sense.

Hi, med student here.
I came across some plots from this article. Unfortunately, I don’t have access to the full version, so the answer to my question may be in there.
These plots show a linear dependence between urine flow (X-axis) and renal clearance (Y-axis) of ethanol and butabarbital. In the case of urea and chloramphenicol, the function is represented by a branch of a hyperbola.

I don’t understand the kinetics in the case of urea. Since urea reaches the lumen by passive diffusion, I would have expected a linear relationship without a plateau. Additionally, considering the reabsorption process, I would have expected a slow increase in clearance at low urine flow due to the higher gradient between the lumen and the plasma.
Where’s the snag?

Hi! I am a Pharmacist in working in R+D of generic drugs. Just wondering if there's any Regulatory Affairs Specialist from the EU (Including the UK) here to make a question about References medicines. Is there an official list with the reference drug for a medicinal product like the FDA's Orange Book (The reference drug is called Reference Listed Drug or RLD). The request is because I need resources to create protocols for Bioequivalence studies (in vivo and biowaivers). I've been looking for this but no result, also I verified in the EMA page and other agencies.

Thanks in advance.

Hello Everybody! I'm currently a High School Senior in the States looking to get into Pharmacology. I was fortunate to get a job as a pharmacy technician for the past year and have fallen in love with the field. Specifically, how medications work and affect the body. However, I also witnessed how exhausting retail pharmacy can be, especially for the pharmacists. Currently, I'm in between Pharmacy(Hospital or residency) and Pharmacology but feel I don't know enough about the Pharmacology pathway.

First, what's the career like for a pharmacologist? Is it purely in a laboratory setting or are there other workplaces available? I've also seen a lot of programs offering what is pharmacology/toxicology. In the workplace, is there a major difference between the two?

Second, can you specialize in a specific field in pharmacology? Can you specialize in something such as neuropharmacology? Would I need a degree in neuroscience as well? Any recommendations for an aspiring pharmacologist going into college?

Hopefully this type of question is allowed here and I appreciate any info or answers!

Hello, my bachelors was in biological anthropology, and I’m currently a biomedical anthropology MS student working in a lab in the pharmaceutical sciences department at my current university in addiction genetics with mouse models. I was wondering if anyone has:

1. any general advice for me on applying to pharmacology PhDs (ex. do you generally reach out to professors, how did you format your SOPs, what considerations did you have for universities on your short list, how did you decide on a university, what did you do if you didn’t get in, etc.)
2. any specific advice/knowledge on the programs I’m listing below (ex. I heard from the chair of pharmacy here that UBuffalo is well-known for pharmacokinetics but obviously it’s not described as such on their website)
3. job prospects for PhD holders going into industry
4. A very specific consideration: I had a dip in my undergraduation transcript around COVID due to untreated mental health issues, but my junior and senior year plus my graduate transcript are all As and Bs. Is it acceptable to mention mental health in my SOPs to explain my grade dip, and if not how do I approach that?

My shortlist I’m considering are:

• MCPHS
• Boston University
• UMich (PIBS)
• Binghamton University
• PennState (Translational Therapeutics)

I originally had UWash, UBuffalo, and Northeastern on the list, but have personal considerations for cutting them. If someone convinces me that my considerations are lesser than the pros, I might apply.

Hi all

I’ve completed my BSc in Pharmacology and my career aspiration is to become a patent attorney within the pharmaceutical field.

I’m going to do an MSc next year but unsure which MSc course to do. Are there any fields within life sciences that you think there will be a strong demand for patent attorney’s in the future? Or MSc’s that will make me an attractive candidate for training contracts?

I’m currently looking at MSc’s in Pharmacology, Immunology, Virology, Stem Cells and Neuroscience.

TIA!

I will be completing my MBBS next year, but instead of pursuing clinical specialties to work in a hospital setting, I am passionate about pharmacology. My goal is to either become a professor in this field or work in the pharmaceutical industry. However, I have realized that many advanced pharmacology programs require a background in mathematics, which I did not focus on during my undergraduate studies. I am seeking guidance on how best to bridge this gap and fulfill the necessary requirements. Specifically in the USA

Hello all, My name is Adrian. I am currently a high school senior currently applying early to colleges.

I am aiming to be a Pharmacist and am trying to set myself up with a solid undergraduate education. I consider myself a fairly versed student as far as my education. The math/science courses I have thus far taken that I would view as concerning pharmaceutical studies are Biology Honors, Geometry Honors, Algebra Honors, AICE Environmental Science, Pre-Calculus Honors, Forensic Science, Chemistry Honors, and I am currently taking AP Biology as well as Calculus Honors.

My biggest concern is in majoring in biology which I had my heart set on for a little bit now due to my interest in the subject as well as the direct correlation it has to my desired career. The problem with biology is that it is highly regarded as one of the hardest majors, though I most definitely see myself as capable (as I am a firm believer that I can do anything I put my mind to), I wonder if I am setting myself up for an unnecessarily stressful undergraduate life. My second choice is to major in psychology which is another longstanding interest that I have had (specifically forensic psychology) and then taking the classes necessary for pharmacy school.

I guess what I need clarity on is:

1. Is majoring in Biology as hard as it is chalked up to be?

2. Does majoring in Biology seem reasonable/feasible for me given the courses I've taken (feel free to ask follow-up questions regarding specific grades, my GPA, etc.)?

3. If you are familiar with pharmacists who majored in Biology how important is it for success in Pharmacy school as far as preparedness (I imagine it isn't integral to success, but did they have a significant edge over those who didn't)?

Thank you in advance for your help and guidance and please feel free to let me know if you have any more advice on the admissions process or anything else you feel may be relevant!

Beloved swarm intelligence, I am currently looking for in vitro and in vivo possibilities to evaluate the impact of a drug on mucociliary clearance. So far the only in vitro assay I found is provided by Epithelix. There are some possibilities in vivo, from measure particle to phenol red clearance. Are there any people out here with experience in any respective in vivo assay, possibly with a recommendation for a CRO offering the assay?

Pramipexole is given as an adjunct alongside irreversible MAOIs. As the title says, why, if mono amine oxidase is being inhibited, would it not affect the production of dopamine in the mesolimbic system from D3 agonism?

I'm a certified pharmacy technician and working in retail pharmacy is absolutely horrible. both the technicians and pharmacists are yelled at daily and I just can't take it anymore as an introvert and as an autistic person.

I absolutely want to stay in medicine but be more into a lab setting where I don't have to interact with the public every single day.

Is pharmacology good for introverts?

I have a question regarding a statement in this article:Toutain PL, Bousquet-Mélou A. Volumes of distribution. J Vet Pharmacol Ther. 2004 Dec;27(6):441-53. doi: 10.1111/j.1365-2885.2004.00602.x. PMID: 15601439 If we assume that no drug has been distributed,then why isn't the concentration fixed since the whole drug is in the blood and the plasma concentration is known(say we have calculated it somehow) I tried explaining this by assuming that the text means no further distribution after a hypothetical instant distribution of the drug,but I'm not confident in this assumption. Thanks for answering in advance .

Hello! I have a question regarding a case study. The patient’s empirical treatment plan lasted for 6 weeks. During the first 2 weeks, she was on a triple therapy consisting of amoxicillin, clarithromycin, and omeprazole. While taking these medications, she discontinued both simvastatin and ibuprofen. After completing the antimicrobial treatment, she resumed taking simvastatin and ibuprofen for the remaining 4 weeks. Why was there a need to stop these medications during the initial phase of treatment?

Additionally, as this regimen eventually proved unsuitable, her omeprazole dose was adjusted to 40 mg once daily, whereas previously she was taking 20 mg twice daily. How does this change affect the pharmacokinetics of the drug?

Hey Everybody!

I've been extremely interested in entering the pharmacology field, and was wondering what undergraduate degrees would get me on track to entering a pharmacology program in the future. I figured it would be best to ask those that have already studied or are currently studying as you often have different insight after going through the experience yourself.

Thank you in advance for any responses, I truly appreciate anyone that takes the time out of their day!

My why: Looking for new direction in life. I was diagnosed with ADHD in second grade, took a million different medications, and I have been obsessed by how pharmaceuticals and chemicals affect the brain ever since I figured out how to use google. Being sort of forced into taking medication at a young age, that I didn’t fully understand, I developed major hatred towards western medicine. Also watched my mother misuse medication which really affected my outlook. This also lead to me leaning into psychedelics, Ayurveda, RC’s, nootropics, a million different types of supplements…. Thank god peptides and SARMs where not a thing when I was a teen. My bias heavily influenced how I gathered my research. I was in sales for 6 years, was an independent contractor and a lot of my colleagues had the entrepreneurial red-pill spirit which I sort of adopted myself. Then became a personal trainer over the last 2 years and caught myself falling even deeper into all the grifters like Hiberman for example. Over the last year I’ve done a lot of debating with friends and colleagues about Ozempic, I’ve kept an open mind and finally learned how to research things properly. This is the final straw for me. I demonized it, and I finally waking up to the reality how important and misunderstood pharma is. I want to be able to have a deeper understanding on how all these things work. I want to be able to defend pharmaceuticals properly for people who actually need them. I want to be able to have a proper stance against so many people spreading misinformation. I want to understand mental illness and pharmaceutical intervention better. I have a tendency to jump from one thing to the next but I find pharmacology extremely stimulating.

My concern: I’m 30. I’ve neglected a lot most my life due to trauma and being in survival mode. I’m completely starting from scratch, went straight into physical labor and sales with no formal education. I don’t mind having to spend another 10 years of my life dedicating myself to a degree before I’d even be eligible for a job within the field.

How plausible is this desire? Is there a path you could recommend?

This is not my area of expertise and your help would be very much appreciated!

I need to estimate a realistic shelf life of the antibody(Fc)-drug(dimeric zanamivir)-conjugate CD388:

https://www.cidara.com/wp-content/uploads/2024/08/Cidara-Corporate-Presentation-August-2024-2.pdf

https://www.biorxiv.org/content/10.1101/2024.06.04.597465v3.full

I'm aware of the shelf life extensions granted to antivirals and antibodies. However the ADCdb doesn't provide shelf life data. Clearly there is a conflict of interests between manufacturers and the U.S. Strategic National Stockpile (SNS). And I can't rule out that ADCs are much less stable than antivirals or antibodies individually.

What is your best guess regarding the shelf life of CD388, assuming that the need and political will for stockpiling exceeds liability issues?

https://www.fda.gov/emergency-preparedness-and-response/mcm-legal-regulatory-and-policy-framework/expiration-dating-extension

https://academic.oup.com/nar/article/52/D1/D1097/7311080

http://adcdb.idrblab.net/search/adc_search

"Tamiflu has a shelf life of 10 years, as established by its manufacturer (Roche, now merged with Genentech) and as mandated by the European Union. But the U.S. Food and Drug Administration has some stock that dates to 2004, and the FDA has extended the shelf life of some of the oldest purchased Tamiflu products to 20 years for emergency responses–a decision that prompted a Genentech spokesperson to distance the company from it."

https://fortune.com/2024/06/24/us-strategic-drug-stockpile-inadequate-bird-flu-outbreak/

"Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12–26 years, and found them to work perfectly on routine histology sections. It is not unreasonable to suggest that our findings relating to the use of antibodies for diagnostic immunohistochemistry may be extended to all fields where antibodies are used as a primary, unconjugated detection layer to tag an antigen. Given the actual pace of new technical developments in diagnostic pathology practice, we have shown that, relatively speaking, antibodies are forever."
Source: Antibodies are forever: a study using 12–26-year-old expired antibodies

Edit:

"Some ADCs may be stored at 4°C for a few weeks or months. Freezer storage of ADCs is generally not recommended due to the possibility of accelerated aggregation or precipitation during the freezing process.

CellMosaic® has developed proprietary ADC stabilizing buffers that can be used for long-term storage of ADCs. The buffers contain stabilizers to prevent the hydrophobic drugs from interacting with one another and keep the ADCs in solution when stored below freezing conditions."

https://www.cellmosaic.com/adc-stabilizing-buffer/

"In conclusion, ADC-stabilizing buffers can slow down the aggregation or precipitation process during storage for antibodies labeled with very hydrophobic drugs. Furthermore, ADC-stabilizing buffers allow the long-term storage of ADCs in solution at less than -20°C or as a lyophilized powder."

https://www.cellmosaic.com/content/Manual/Application%20Notes/AN201901_R1_final3.pdf

Singh, S. K., Luisi, D. L., & Pak, R. H. (2015). Antibody-Drug Conjugates: Design, Formulation and Physicochemical Stability. Pharmaceutical Research, 32(11), 3541–3571. doi:10.1007/s11095-015-1704-4 

https://sci-hub.se/10.1007/s11095-015-1704-4

Large-Scale Freezing of Biologics

https://www.researchgate.net/publication/286396243_Large-Scale_Freezing_of_Biologics

Physical and Chemical Stability of Antibody Drug Conjugates: Current Status

https://www.jpharmsci.org/article/S0022-3549(15)00152-5/fulltext

Optimization of Drug-Linker to Enable Long-term Storage of Antibody–Drug Conjugate for Subcutaneous Dosing

https://pubs.acs.org/doi/10.1021/acs.jmedchem.3c00794

Hi, I'd like to be able to make fully-informed decisions regarding drugs/supplements/etc that I take. I'm especially interested in nootropics.

Only reading studies, and otherwise learning randomly, would lead to a lot of confusion. That's why I'm looking for resources that could help me get started with a structured approach that shows how everything connects together; the medium can be anything, whether it be books, courses, or even podcasts. I'd also appreciate recommendations of pop-sci books, so that I have something to read/ listen to while tired and otherwise incapable of experiencing more advanced material.

Thank you

I'm interested in having a look at the initial stability studies Pfizer presumably performed to establish the shelf life for mycobutim (rifabutin). How would I go about finding those? If they are not published and indexed by databases (as I assume may be the case), would they be available on websites of regulatory agencies or perhaps upon request from the agencies or Pfizer?

As I'm sure most of you know, there's been a real problem with generics of various stimulant medications. You can argue they are bio-equivalent but that's not what I'm asking. I read somewhere that there was a shortage of active ingredients or something. Not just a shortage of medication, but something required to make it. I couldn't find any follow up on what elements used in these medications are in short supply and why.

There are two 'enatiomer' amphetamine and four different 'salts' that may be mixed. Is it possible they aren't able to make these the way they did before because they don't have access to an ingredient needed for the process?

What are the ingredients in these formulas/process that they could be having trouble getting ahold of to make these medications properly causing the dramatic reduction in efficacy?

Hi everybody, I stumbled upon this OT-2 robot that a research lab I used to work for used for automated pipetting, but I am wondering if this robot can also be used in pharmaceuticals (process analytics) for automatization of sample preparation (for example before HPLC/MS or other analytical methods used primarily in pharmaceuticals).

All info will be highly appreciated!

Hello, I have my PhD in Pharmacology & toxicology. I am currently working in policy but I have been curious about other career paths. Can anyone share their journey on becoming a Surgical or clinical pharmacologist? Also, can you share your general day to day work?

Thank you

I see PRN MiraLAX ordered all the time in lieu of faster acting agents like bisacodyl or magnesium hydroxide. When I look up the kinetics, MiraLAX has an onset of action up to 4 days.

What’s the rationale behind PRN MiraLAX instead of other faster acting agents?

Hi!

Short : looking for books to give better recommandations to patients

Long : I was graduated in 2018 as pharmacist in France, and chose to work in pharmaceutical industry for 6 years (QA, production). I decided few months ago to finally work as pharmacist in a pharmacy (first as employee, and I hope to become an owner one day).

I didn't really keep all my courses, and I forgot a lot about them. What books would you recommend to go "back on track" in order to give better advices to patients? I just ordered a pharmacology book to get a better understanding of medicines interactions (rand&dale, based on Reddit recommandations), but I was wondering if you had any other book recommendations? (Advices, plant based therapy...)

Thank you all !

Regarding dosing of omeprazole (Prilosec) - I’m aware usual dosing is once daily, e.g. 20-40mg (depending on indication). Given that half-life is approx 3 hrs, why is the dosing not divided during the day, e.g. 20mg morning/20mg evening (if on a total 40mg dose).

Hi I work for a biotech and we need a poppk model with exposure response analysis created. We went to a major vendor and they were giving a timeline of 4-5 months. Why should such an analysis take that long? We have to like 6 studies to incorporate into eh analysis but 3 months...?

Anyone work in modeling and can explain what could drive such a slow turnaround?

Which of the following is NOT a factor affecting drug metabolism?

• A) Age of the patient
• B) Drug interactions
• C) Route of administration
• D) Presence of food in the stomach

Since the route of administration can affect on the bioavailability of the drug?

Vonoprazan is increasingly being used with various combinations of antibiotics for H. pylori therapy and will presumably more and more replace PPIs. Currently, most guidelines recommend bismuth quadrouple therapy as first line treatment for H. pylori. Very little of bismuth is absorbed when taken orally, but its bioavailability increases as intragastric pH increases and with PPIs being replaced by the more potent vonoprazan, there could be risk of excessive bismuth absorption.

I looked into whether any studies were done on this and found this one which measured serum bismuth levels following the administration of two bismuth containing regimens, one with 20mg esomeprazole bid, one with 20mg vonoprazan bid and here's what they found on day 14 of dosing:

The mean plasma bismuth concentration increased from 6.98 ng/mL (before dosing) to 47.00 ng/mL in the vonoprazan group and from 6.38 ng/mL (before dosing) to 35.47 ng/mL in the esomeprazole group at 0.75 h after dosing.

Now, the authors present this in a terribly blase fashion and conclude the regimen is safe, but the generally accepted limit for bismuth toxicity is 50 ng/mL. The 47 ng/mL value they give is the mean, meaning some of the subjects had concentrations higher than that.

Now, granted, the concentration drops fairly quickly after administration, still I found it a bit alarming. Do you think there's cause for concern here?